免疫抑制剂干扰联合瘤细胞接种构建肝癌移植瘤小鼠模型

The establishment of transplanted hepatocellular carcinoma by immunosuppressor interference and inoculation with tumor cells

目的探讨构建肝癌移植瘤动物模型的最佳方法。方法选用BALB/c小鼠160只,随机分为地塞米松组、肝癌细胞注射组、地塞米松加肝癌细胞注射组及空白对照组,每组40只,雌、雄各半。地塞米松组在小鼠腹腔内注射地塞米松;肝癌细胞注射组于不同时间在肝脏直接接种H22肝癌细胞株3次;地塞米松加肝癌细胞注射组将上述2种方法联合使用;空白对照组注射生理盐水。1个月后,颈椎脱臼处死小鼠,取其肝组织,计算肝指数,苏木精-伊红染色(HE)后观察肝组织病理变化。结果地塞米松组、空白对照组未发现肝组织癌变,肝癌细胞注射组、地塞米松加肝癌细胞注射组癌变的百分比分别为71.4%(20/28)、91.2%(21/23)。结论肝癌细胞直接接种小鼠肝脏及联合地塞米松干扰均可建立肝癌移植瘤动物模型,但联合地塞米松干扰建模的成功率明显升高。

Objective To explore the optimum method of establishing hepatocarcinoma-bearing mice′s model of trans-plantation tumor. Methods Totally 160 BALB/c mice were selected and randomized into dexamethasone group ,hepatoma carci-noma cell injection group,dexamethasone and hepatoma carcinoma cell injection group and blank control group,40 cases in each group and sex in half. The dexamethasone group was with intraperitoneal injection of dexamethasone;the hepatoma carcinoma cell injection group was directly inoculated hepatoma carcinoma cell line H22 in liver three times at different times;the dexamethasone and hepatoma carcinoma cell injection group combined with the above two methods and the blank control group was injected with normal saline. One month later,the mice were killed by cervical dislocation to extract liver tissue,calculate the liver index and ob-serve the pathological changes of liver tissue after hematoxylin-eosin（HE） staining. Results The cancer was not found in dex-amethasone group and control blank group. The percentage of carcinogenesis in liver tissue injection group ,dexamethasone and hepatoma carcinoma cell injection group was 71.4%（20/28） and 91.2%（21/23） respectively. Conclusion Hepatocarcinoma-bearing mice′s model of transplantation tumor can be established by inoculating mice hepatoma carcinoma cell directly and interfer-ence of dexamethasone combined with cyclophosphamide ,moreover combination with dexamethasone interference can improve the success rate of modeling significantly.