硫酸镁对人脐静脉血管内皮细胞辐射防护的作用

Protective effects of MgSO_4 on the human umbilical vein endothelial cell injury induced by radiation

将对数生长期的人脐静脉血管内皮细胞(Human umbilical vein endothelial cell,HUVEC)分为对照组、照射组和实验组,照射组和实验组均接受γ-射线辐照,实验组于照射前0.5 h加入硫酸镁。采用CCK-8法检测细胞存活率,流式细胞仪检测细胞周期分布和凋亡率,硫代巴比妥酸法检测丙二醛(MDA)水平。结果显示,6.25mg·mL-1以下浓度的硫酸镁对HUVEC细胞存活率无明显影响;6.25 mg·mL-1的硫酸镁可提高照射后HUVEC细胞的存活率,改善γ-射线诱导的HUVEC细胞G2/M期阻滞,降低HUVEC细胞凋亡率。提示硫酸镁对HUVEC细胞辐射损伤具有一定的防护作用。

The human umbilical vein endothelial cells （HUVEC） under exponential growth phase were divided into three categories： normal group, irradiation group, and experimental group, where the irradiation group was only treated with γ-ray, and the experimental group was incubated with MgSO4 0.5 h before being treated with γ-ray. Then the HUVEC was collected at the corresponding time to be researched. The CCK-8 method was adopted to detect the cells＇ survival rate, the flow cytometry was used to measure the cells＇ distribution cycle and apoptosis rate, and the thiobarbituric acid （TBA） method was employed to test the level of malondialdehyde （MDA） included in HUVEC. The experimental results show that the MgSO4 with concentration below 6.25 mg.mL-1 was non-toxic to HUVEC. However, the MgSO4 with concentration of 6.25 mg.mL-1 can not only improve the survival rate of the HUVEC treated with γ-ray, but also alleviate the cells＇ G2/M arrest cycle and reduce the cells＇ apoptosis rate. Therefore, the MgSO4 has certain protective effect on the HUVEC with radiation injury.