急性病毒性心肌炎患儿血中VCAM-1及IL-6的浓度变化及其相关性研究

THE CHANGES OF VCAM-1AND IL-6 IN CHILDREN WITH ACUTE VIRAL MYOCARDITIS AND THEIR MUTUAL RELATIONS

目的:检测急性病毒性心肌炎患儿外周血中血管细胞黏附分子-1(VCAM-1)、白介素-6(IL-6)的浓度并分析其相关性,探讨其在急性病毒性心肌炎发病机制中的部分作用。方法:选取2008-09到2011-09在内蒙古医科大学附属医院儿科临床诊断急性病毒性心肌炎患儿30例,为实验组。健康儿童30名,为对照组。采用实验研究策略,引入双抗体夹心ELISA法,检测两组外周血中的VCAM-1、IL-6的浓度,并进行分析比较。结果:实验组VCAM-1的血清含量为1385.467±27.664μg/L,比对照组的787.16±86.79μg/L明显增高,差异有统计学意义(t=35.980,P=0.000<0.01);实验组IL-6血清含量为107.26±11.42ng/L,与对照组的57.49±9.45ng/L明显增高,差异有统计学意义(t=18.389,P=0.000<0.01)。实验组IL-6和VCAM-1血清含量分析显示呈密切正相关(r=0.981,P=0.000<0-01)。结论:急性毒性心肌炎发病机制中有VCAM-1、IL-6的参与,提示细胞因子及免疫应答在其的发生发展中有重要作用。

Objective：To detect the concentration of IL-6 and VCAM-1 in children with acute viral myocarditis, and explore the part effect of IL-6 and VCAM-1 on acute viral myocarditis. Methods：Experimental study 30 cases of children have been clinical diagnosed with viral myocarditis at the acute stage were chosen as the experimental group, with 30 cases of healthy children as the controlled group. By means of double-antibody sandwich ELISA methed,the concentration of IL-6 and VCAM-1 were measured in both the experimental group and controlled group. Results：The valus of IL-6 at the experimental group（107. 42±11. 42） ng/L were remarkable increased,compared with the controlled group（57.49±9.45）ng/L. The differences were statistically significant（t=18.389. P=0 . 000〈0 . 01 ） . The valus of VCAM-1 at the experimental group （ 1385-467±27 . 664 ） ug/L were remarkable increased,compared with the controlled group（787. 16±86. 79）ug/L;The differences werestatistically significant（ t=35. 980, P=0. 000〈0. 01 ）;The concentration of IL-6 being positively related to VCAM-1 at the experimental group. Conclusion：The pathogenesis of viral myocarditis involves IL-6 as well as VCAM-1 participation. Cell-mediated immunity and cytokines play an important role in the development of viral myocarditis.