PSMA7对A549细胞表面黏附分子表达的影响

Effects of PSMA7 changes on adhesion molecule expression on A549 cell

目的探讨PSMA7表达量的变化对A549细胞表面黏附分子ICAM-1,VCAM-1和CD44表达的影响。方法采用流式细胞仪分别检测高表达PSMA7的A549细胞[pcDNA3.1(-)/PSMA7组]和低表达PSMA7的A549细胞(shPSMA7组)表面黏附分子ICAM-1,VCAM-1和CD44的表达情况。结果与高表达阴性对照组[pcDNA3.1(-)组]相比,pcDNA3.1(-)/PSMA7组的ICAM-1(t=86.325,P=0.000)和VCAM-1(t=16.337,P=0.000)的表达量均显著降低,CD44表达量则无明显变化(t=-0.545,P=0.615),而与低表达阴性对照组(shNC组)相比,shPSMA7组ICAM-1(t=-119.827,P=0.000)和VCAM-1(t=-26.68,P=0.000)表达量均显著增高,CD44表达量则无明显变化(t=-848,P=0.444)。与pcDNA3.1(-)组相比,pcDNA3.1(-)/PSMA7组穿膜侵袭细胞的数量明显减少(t=3.553,P=0.024),而shPSMA7组明显高于shNC组(t=-3.207,P=0.033)。结论高表达或干扰PSMA7可影响A549细胞表面黏附分子ICAM-1,VCAM-1的表达,提示PSMA7可能因此参与肺腺癌细胞的侵袭和转移。

To observe the effect of PSMA7 changes on adhesion molecule expression on A549 cell, flow cytometry was used to detect the expression of ICAM-1, VCAM-1 and CD44 in pcDNA3.1（-）/PSMA7 A549 cell line and shPSMA7 A549 cell line. Compared with pcDNA3.1（-） group, the expression of ICAM-1 （t=86.325, P= 0.000） and VCAM-1 （t=16.337, P=0.000） in pcDNA3.1（-）/PSMA7 group was significantly decreased, but CD44 expression （t=-0.545, P=0.615） did not change; compared with shNC group, the expression of ICAM-1 （t=-119.827, P=0.000） and VCAM-1 （t=-26.68, P=0.000） in shPSMA7 group was significantly increased, but CD44 expression （t=-848, P=0.444） did not change; compared with pcDNA3.1（-） group, the number of migrated A549 cells in pcDNA3.1（-）/PSMA7 group was significantly decreased （t=3.553,P=0.024）; and compared with shNC group, migrated A549 cells of shPSMA7 group significantly increased （t=-3.207,P=0.033）. In conclusion, over- and low- expression of PSMA7 maybe affect the expression of adhesion molecule ICAM-1 and VCAM-1 in A549 cell line.