胃癌组织EB病毒感染与miR-101 EZH2 COX-2表达关系的研究

Correlation of Epstein-Barr virus infection with the expression of miR-101, EZH2, and COX-2 in gastric cancer

目的:探讨甘肃省武威地区胃癌患者EB病毒(Epstein-Barr virus,EBV)感染状况及miR-101、EZH2、COX-2在EBV相关胃癌发生发展中的作用。方法:应用组织芯片、原位杂交和免疫组织化学技术检测120例胃癌组织及相应癌旁组织中EBV小RNA(EBER)、miR-101、EZH2、COX-2的表达情况。结果:120例胃癌组织EBV阳性率为10.0%,EBV相关胃癌有较少的淋巴结转移,好发于贲门、胃体(P<0.05)。miR-101、EZH2、COX-2在120例胃癌组织和相应癌旁组织的阳性率差异有统计学意义(P<0.05)。12例EBV阳性胃癌组织miR-101、EZH2、COX-2和108例EBV阴性胃癌组织3个分子的表达率差异有统计学意义(P<0.05)。胃癌组织EBV感染和miR-101表达呈正相关,EBV相关胃癌组织miR-101表达和淋巴结转移、COX-2、EZH2表达均呈负相关(P<0.05)。结论:EB病毒感染与武威地区胃癌的发生有一定关系;EBV相关胃癌和EBV阴性胃癌在淋巴结转移和发生部位的差异有统计学意义;miR-101、EZH2、COX-2与EB病毒相关胃癌的发展有一定关系。

Objective：This study was conducted to investigate Epstein-Barr virus （EBV） infection in gastric cancer in Wuwei ar-ea of Gansu province （China） and the roles of miR-101, EZH2, and COX-2 in EBV-associated gastric carcinomas （EBVaGC）. Meth-ods：Tissue microarray technique, immunohistochemistry, and in situ hybridization were performed to detect the expression of EBV-en-coded small RNAs （EBERs）, miR-101, EZH2, and COX-2, in gastric cancer tissues （n=120） and the corresponding adjacent tissues （n=120）. Results：The positive rate of EBV was 10.0% in 120 cases of gastric cancer tissues. EBVaGC was not significantly associated with lymph node metastasis and developed most often in the cardia and body （P＆lt;0.05）. The differences in the positive rates of miR-101, EZH2, and COX-2 in 120 cases of gastric cancer tissues and corresponding adjacent tissues were significant （P＆lt;0.05）. The differences in the positive rates of miR-101, EZH2, and COX-2 in 12 cases of EBVaGC and in 108 cases of EBV-negative gastric can-cer （EBVnGC） tissues were significant （P＆lt;0.05）. The infection of EBV in gastric cancer tissues was positively related to miR-101 ex-pression. By contrast, miR-101 expression was negatively related to lymph node metastasis and expression of COX-2 and EZH2 in EB-VaGC tissues （P＆lt;0.05）. Conclusion：EBV infection was related to gastric cancer in Wuwei area of Gansu province. EBVaGC and EB-VnGC have significant differences in lymph node metastasis and in the location of cancer. MiR-101, EZH2, and COX-2 were related to the development of EBVaGC.