白介素-2信号通路在鼻息肉发病中的作用

The role of Interleukin-2 pathway in pathogenesis of nasal polyps

目的:研究白介素2(IL-2)信号通路相关因子及调节性T细胞在鼻息肉患者组织中的表达情况,探讨二者之间的关联及其在息肉发病中的可能作用机制。方法:集取鼻息肉及正常对照组共30例患者的息肉组织及鼻甲黏膜,采用ELISA法检测局部组织内IL-2及IL-2R,免疫印迹检测组织中的磷酸化STAT5,实时荧光定量PCR法检测Foxp3mRNA在局部组织的表达情况;采用流式细胞术检测局部组织内Treg细胞的比率;同时分析IL-2通路相关因子及Treg细胞的相关性。结果:鼻息肉患者局部组织中IL-2、IL-2R及pSTAT5的含量较正常对照组显著降低,差异有统计学意义(均P<0.05);同时,与正常对照组相比,鼻息肉患者的Foxp3mRNA及Treg细胞的比率显著下调,差异有统计学意义(均P<0.05);且鼻息肉局部组织内IL-2信号通路相关因子的表达与Treg细胞比率及Foxp3mRNA水平呈正相关(均P<0.01)。结论:IL-2信号通路的活化状态在鼻息肉患者局部组织中发生了变化,且与Treg细胞的表达呈正相关,提示IL-2信号通路在鼻息肉的发病中起重要作用,鼻息肉中Treg细胞的下调可能是由IL-2信号通路的活化下调所引起的。

Objective：To study the expression of Interleukin-2 （IL-2） signaling pathway related factors and Treg cell in nasal tissue of nasal polyps, so that to investigate the possible mechanism of IL-2 signaling pathway in the progress of nasal polyps and the correlation between IL-2 pathway and Treg cell. Method：Thirty ＇patients were enrolled for study, including the patients with nasal polyps and those with only deviated nasal septum as normal control. The nasal polyps tissue and the turbinate mucosa of the patients were collected during surgery. The ex- pression levels of IL-2 and IL-2R were measured by means of EI.ISA. The level of pSTAT5 was evaluated by Western blot. We measured the level of Foxp3 mRNA in the tissue by real-time PCR, and the proportion of Treg cells was evaluated by flow cytometry. Finally, we analyzed the correlation between IL-2 pathway related factors and Treg cells in nasal polyps. Result： The expression levels of IL-2, IL-2R and pSTAT5 were significantly de- creased in the nasal polyps compared with normal control （P〈0. 05）, and the level of Foxp3 mRNA and propor- tion of Treg cells in patients with nasal polyps were significantly lower than in normal control （P〈0. 05）. Moreo- ver, there was positive correlation between the levels of IL-2 pathway related factors and the levels of Foxp3 mR- NA and Treg cells proportion in nasal polyps. Conclusion： The activated state of IL 2 signaling pathway got changed in nasal polyps tissue, the level of which was positively correlated with the expression of Treg cells, indi- cating that the IL-2 signaling pathway may play a crucial role in the development of nasal polyps, and the de- creased level of Treg ceils in nasal polyps may result from the downregulation of IL-2 signaling pathway.