摘要
目的:研究雷公藤红素对大鼠变应性鼻炎(AR)的治疗作用并初步探讨相关作用机制。方法:构建大鼠AR模型,测定大鼠第1天、第4天和第7天的行为学特征改变、鼻黏膜呼吸区组织超氧化物歧化酶(SOD)、丙二醛(MDA)、还原型谷胱甘肽(GSH)和谷胱甘肽过氧化物酶(GSH-PX)的水平;测定鼻黏膜呼吸区组织核因子E2p45相关因子2(NRF2)的核内蛋白表达及谷氨酸半胱氨酸连接酶催化亚基(GCLC)的浆蛋白表达。结果:AR大鼠第1、4、7天的行为学特征改变显著(P<0.01),鼻黏膜呼吸区组织SOD、GSH、GSH-PX降低,MDA升高;NRF2的核内蛋白表达降低,GCLC的浆蛋白表达降低。雷公藤红素低剂量治疗组和高剂量治疗组能不同程度改善上述指标的变化,同时提高了鼻黏膜呼吸区组织NRF2的核蛋白表达和GCLC的浆蛋白表达。结论:雷公藤红素能改善AR大鼠的症状,其机制可能与提高鼻黏膜呼吸区组织NRF2的核蛋白表达和GCLC的浆蛋白表达相关。
Objective：To investigate the protective effect of celastrol on allergic rhinitis rats and its possible mechanism. Method：Allergic rhinitis （AR） model of rats was established by OVA. The behavioural characteris- tics were observed at the 1st, 4th and 7th dayafter stimulation treatment. The levels of superoxide dismutase （SOD）, malondialdehyde （MDA）, glutathione （GSH） and glutathione peroxidase （GSH-PX） in the nasal mucosa breathing zone were measured. The expression of the nuclear factor erythroid 2 related factor 2 （NRF2） nuclear protein and the catalytic submit of glutamylcysteine ligase （GCLC） cytoplasmic protein in the nasal mucosa breath- ing zone were determined. Result：We observed obvious behaviour changes related with allergic rhinitis in AR rats, together with decrease of SOD, GSH and GSH-PX and increase of MDA in the nasal mucosa breathing zone. Mo- reover, NRF2 nuclear protein expression and GCLC cytoplasmic expression were suppressed in the nasal muucosa. The changes above were alleviated in celastrol pretreatment group. The potential mechanism may be related to the upregulation of NRF2 nuclear protein expression and GCLC cytoplasmic expression after celastrol pretreatment. Conclusion：Celastrol can significantly relieve the allergic symptoms in AR rats. The mechanism of this protective effects may relate to the upregulation of NRF2 nuclear protein expression and GCLC cytoplasmic expression in the nasal mucosa breathing zone.
出处
《临床耳鼻咽喉头颈外科杂志》
CAS
北大核心
2014年第8期550-553,共4页
Journal of Clinical Otorhinolaryngology Head And Neck Surgery
