人前梯度蛋白2在结肠癌组织中的表达及其意义

Clinical signifi cance of expression of anterior gradient-2 in colon adenocarcinoma

目的:检测人前梯度蛋白2(anterior gradient-2,AGR2)在结肠癌组织中的表达及其与结肠癌临床病理参数之间关系.方法:采用半定量反转录-聚合酶链反应(semiquantitative RT-PCR)检测结肠癌及癌旁正常组织中AGR2 mRNA表达情况,应用蛋白质印迹(Western blot)、免疫组织化学S-P法检测结肠癌及癌旁正常组织中AGR2蛋白的表达情况,并结合临床病理参数进行分析.结果:半定量PCR及Western blot结果显示,AGR2 mRNA及蛋白在结肠癌的表达水平均明显高于癌旁正常组织(mRNA:0.95±0.03vs 0.21±0.06,P<0.05;蛋白:0.93±0.03 vs0.31±0.02,P<0.05);免疫组织化学结果显示,AGR2在结肠癌组织中阳性表达率明显高于癌旁正常组织(75%vs 29.4%,P<0.05),AGR2在结肠癌组织中的表达与年龄、性别无相关性(P>0.05),与Dukes分期、组织学分级、淋巴结转移均显著相关(P<0.05).结论:AGR2在结肠癌组织中的表达上调可能与结肠癌的发生、发展、转移相关,有望成为结肠癌的早期诊断指标.

AIM： To investigate the expression of anterior gradient-2 （AGR2） in colon adenocarcinoma and the relationship between AGR2 expression and clinicopathological features of colon adenocarci- noma. METHODS： AGR2 mRNA and protein expres- sion in colon adenocarcinoma and tumor- adjacent non-cancerous tissues was detected by semi-quantitative RT-PCR, Western blot and im- munohistochemistry. RESULTS： The expression of AGR2 mRNA and protein in colon adenocarcinoma was sig- nificantly higher than that in tumor-adjacent non-cancerous tissues （0.95 ± 0.03 vs 0.21 ± 0.06, 0.93 ± 0.03 vs 0.31 ± 0.02, P 〈 0.05 for both）. Thepositive rate of AGR2 expression in colon ad- enocarcinoma was significantly higher than that in tumor-adjacent non-cancerous tissues （75% vs 29.4%, P 〈 0.05）. The expression of AGR2 was correlated with Dukes stage, histopathological grade and lymph node metastasis （P 〈 0.05 for all）, but not with other clinicopathologic factors. CONCLUSION： Our findings indicate that the expression of AGR2 is closely related to the tumorigenesis, progression and metastasis of colon adenocarcinoma. AGR2 may be used as a diagnostic marker for colon adenocarcinoma.