复合酶协同微波处理提取紫背天葵叶片总黄酮工艺

Complex Enzyme and Microwave Assisted Extraction Technology of Total Flavonoids from Begonia fimbristipula Hance Leaves

目的:以紫背天葵叶片为原料,研究复合酶(纤维素酶和果胶酶)协同微波处理提取紫背天葵叶片中黄酮类物质,以及每个叶期不同叶片中黄酮类物质提取的工艺条件。方法:采用单因素及正交试验对复合酶水解后微波提取与直接微波提取总黄酮的效果进行对比试验。结果:将紫背天葵叶片50℃烘干,粉碎过80目筛,料水比1∶15(W∶V),加入0.2%果胶酶和0.05%纤维素酶,55℃水解40 min,再加入体积分数60%乙醇作提取剂,料液比1∶30,微波功率360 W,温度70℃下处理20 min后,所得浸提液中总黄酮含量比直接微波处理提高近30%。紫背天葵不同叶期、不同叶片黄酮类物质含量的规律为展开叶和成熟叶中总黄酮含量较高,幼叶次之,老叶中最低。结论:优化的工艺简便可行,提取率高,为紫背天葵黄酮类物质提取工艺的产业化提供理论依据。

Objective： With the Gynura bicolor leaves as raw material, to study on the composite enzyme（cellulose enzyme and pectinase） collaborative microwave processing to extract flavonoids from Gynura bicolor leaves, and the process conditions of flavonoids from leaves of different leaf in each period. Method： Using single factor and orthogonal test to contrast the effect of microwave extraction of flavonoids after composite enzyme hydrolysis and microwave extraction of total flavonoids directly. Results： The content of total flavonoids in leaching liquid increased about 30% than the direct microwave treatment as the condition of Gynura bicolor leaves in 50 ℃ drying, smashing through a 80 mesh sieve, the ratio of material to water 1∶15（W∶V）, adding 0.2% pectinase and 0.05% cellulose, hydrolyzing 40 min in 55 ℃, then add 60% ethanol as extracting agent, the ratio of material to liquid is 1 ∶ 30, microwave power of 360 W, treatment of 20 min in 70 ℃. The content of total flavonoids in Gynura bicolor leaves is higher in expanded leaves and mature leaves, less in young leaves, and the lowest in the old leaves. Conclusion： The optimized process is simple and feasible, and has high extraction efficiency, it provides a theoretical basis for the extraction process of flavonoids in Gynura bicolor for industrialization.