血清乙型肝炎病毒前S1及前S2抗原与HBV-DNA的相关性研究

Correlative research between Pre-S1Ag,Pre-S2Ag and HBV-DNA in sera of patients with hepatitis B

目的:了解血清乙型肝炎病毒(HBV)前S1(Pre-S1)抗原与前S2(Pre-S2)抗原与HBV-DNA的相关性。方法:采用酶联免疫吸附法(ELISA)检测320例乙肝患者血清Pre-S1抗原与Pre-S2抗原,同时采用荧光定量PCR方法检测血清HBV-DNA水平。结果:199例HBV-DNA阳性的乙肝患者Pre-S1抗原和Pre-S2抗原的阳性率分别为82.9%和75.9%;121例HBV-DNA阴性的乙肝患者Pre-S1抗原和Pre-S2抗原的阳性率分别为35.5%和26.4%。HBV-DNA阴性组和HBV-DNA阳性组患者的Pre-S1抗原及Pre-S2抗原阳性率均差异有统计学意义(均P<0.01),且Pre-S1、Pre-S2抗原阳性率与HBV-DNA载量呈高度相关性。结论:Pre-S1与Pre-S2抗原是病毒感染和复制的指标,与HBV-DNA具有高度相关性,对临床标本进行HBV-DNA与Pre-S1、Pre-S2抗原联合检测,可弥补HBV-DNA检测的不足,对预防HBV复制和传播有重要价值。

Objective： To analyze the correlation between hepatitis B virus DNA（HBV-DNA） and Pre-S1 Pre-S2 of hepatitis B. Method：Serum Pre-S1 ＆ Pre-S2 from 320 cases were detected by enzyme-linked immunosor- bent assay （ELISA）,and HBV-DNA was detected by fluorescent quantitative PCR at the same time. Result： A- mong 199 cases of HBV-DNA positive patients,the positive rate of Pre-S1 and Pre-S2 were 82.90% and 75.9%,re- spectively. However,among 121 cases of HBV-DNA negative patients,the positive rate of Pre-S1 and Pre-S2 were 35.5 % and 26.4 % ,respectively. There were differences in the positive rate of Pre-S1 and Pre-S2 between HBV- DNA negative patients and HBV-DNA positive patients（P〈0. 01） ,and Pre-S1 and Pre-S2 were highly correlated to HBV-DNA load. Conclusion： Pre-S1 and Pre-S2, as the index to virus replication and infection, may be highly correlated to HBV-DNA load. Determination of HBV-DNA and Pre-S1 ＆ Pre-S2 could make up the lack of HBV- DNA testing,and be of significant value in preventing HBV replication and transmission.