RP-HPLC法测定穿龙薯蓣总皂甙中薯蓣皂甙元的含量

RP-HPLC Determination of Diosgenin in Total Saponin from Dioscorea Nipponica Mak.

目的 :建立反相高效液相色谱法测定穿龙薯蓣总皂甙中薯蓣皂甙元的含量。方法 :采用 μBondapakC1 8柱 ,甲醇为流动相 ,流速 1 .0 ml/min,996二极管阵列检测器 ( DAD) ,检测波长为 2 0 8nm,对穿龙薯蓣总皂甙中薯蓣皂甙元含量进行测定。结果 :能将薯蓣皂甙元与其主要杂质分离开来 ,薯蓣皂甙元在 1 .90～ 9.51 μg范围内峰面积与其浓度线性关系良好 ( r=0 .9985) ,样品平均回收率为 96.1 5% ,精密度 RSD为 1 .72 % ( n=5)。结论 :该法测定样品无需要衍生化 ,分离效果好 ,分析快速准确。

AIM: To develop the method of the determination of total saponins in \%Dioscorea nipponica\% Mak. by reversed phase high performance liquid chromatography. METHOD: This method used μ Bondapak C 18 column with methanol as mobile phase, 1.0 ml/min as flow and 996 photodiode array detector at 208 nm. RESULTS: It can separate diosgenin and its primary impurity. The calibration curve was linear ( r=0.998 5 ) in the range of 1 90~9.51 μg for diosgenin. The average recovery and precision (RSD) for diosgenin were 96.15%( n=5 ) and 1 72%( n=5) respectively. CONCLUSION: Using the method derivatization is not needed in determining sample and the result of separation is good. The analysis is accurate, and satisfactory.