维持性血液透析患者IL-17和IL-23的水平变化及意义

Exploration of IL-17 amount and its correlation to IL-23 in patients with MHD

目的:通过观察维持性血液透析(Maintainance hemodialysis,MHD)患者体内白细胞介素17(Interleukin17,IL-17)和白细胞介素23(Interleukin23,IL-23)水平、相互之间的相关性,探讨IL-17和IL-23的水平变化及其临床意义。方法:采集10例健康体检者作为正常对照组,20例慢性肾衰竭终末期新进入透析患者的外周血。酶联免疫吸附法(ELISA法)检测所有血清样本的IL-17和IL-23水平,逆转录-聚合酶链反应(RT-PCR)检测外周血淋巴细胞中IL-23mRNA表达水平。结果:(1)MHD患者血清IL-17、IL-23以及外周血淋巴细胞中IL-23mRNA水平明显高于正常对照组(P<0.01),且第16次透析前IL-17、IL-23、IL-23mRNA表达高于第4次透析前(P<0.01);(2)MHD患者血清IL-17表达和IL-23、IL-23mRNA表达呈正相关(r=0.743,P<0.01;r=0.575,P<0.01)。结论:IL-17和IL-23的高表达可能参与了MHD患者的慢性炎症形成过程,两者表达水平有密切相关性。

Objective：To observe the changes of IL-17 and IL-23 in patients with maintainance hemodialysis （MHD） for exploring the relation between them and clinical significance Methods：Ten sera from normal people and twenty sera from patients hemodialyzed for more than one week were sampled.Serum IL-17 and IL-23 were measured in all the sera with sandwich ELISA.Reversing transcription-polymerase chain reaction （RT-PCR） detection of IL-23mRNA expression in peripheral blood lymphocytes.Results：（1）Serum IL-17,IL-23 and IL-23mRNA in patients were much higher than those in normal group（P ＜ 0.01）,and serum IL-17,IL-23 and IL-23mRNA before 16th hemodialysis were higher than those before 4th hemodialysis （P ＜0.01）.（2）IL-17 was positively correlated to IL-23 （r =0.743,P ＜ 0.01） and IL-23mRNA （r =0.575,P ＜ 0.01）.Conclusion：IL-17 and IL-23 in patients is much higher than that in normal people,IL-17 are positively correlated to IL-23,IL-23mRNA.Suggesting that IL-17 and IL-23 play an important role in pathogenesis of immune anomaly and micro-inflammation in patients with MHD.