摇晃蛋白引起神经元分枝并促进树突发育

Reelin promoted branching and dendritic development of neurons

目的:研究摇晃蛋白与神经元迁移和发育的关系。方法:通过子宫内电击转染质粒pCAG-GFP到胚胎小鼠大脑皮质,标记迁移中的单个神经元。通过免疫组化染色,观察位于大脑皮质不同层中迁移和分化神经元的形态,统计分析神经元顶突起长度与神经元分枝的关系。结果:摇晃蛋白局限性分布于发育中大脑皮质的边缘带。当神经元顶突起接触到含摇晃蛋白的边缘带后,神经元顶突起缩短并开始出现分枝,而且,神经元的剩余顶突起的长度与总的分枝长度呈极显著负相关,且与分枝点的数目呈极显著负相关。结论:摇晃蛋白促进神经元顶突起的分枝并与树突的发育有关。

Objective：To study the effect of Reelin on neuronal development.Methods：The plasmid pCAG-GFP was transfected into the cortex of embryonic mouse via in utero electroporation to label the migrating neuron.After immunohistochemical staining,the morphology of neurons in different positions of the cerebral cortex were observed.The relationship between the length of leading process and the branch length and number of neurons was analyzed.Results：Reelin was restrictedly expressed in the marginal zone of cerebral cortex.After the leading process of neurons contacted with marginal zone containing Reelin,the leading processes became shorter and shorter and gave rise to more and more branches.The branch length was inverse correlated with the length of the residual leading process.A significant negative correlation between the number of bifurcations and the length of the residual leading process was also found.Conclusion：Reelin promotes the branching of neurons and relates to neuronal dendritic development.