番茄离体再生系统的建立及褐化现象调控研究

Research on Establishment of in vitro Regeneration System of Tomato and Control of Browning Phenomenon

本试验的目的是建立一套完整的番茄体外组培繁殖体系,并对组培过程中容易出现的褐化现象进行控制性研究。结果表明:诱导愈伤组织的最适外植体为子叶,其最适植物生长调节剂浓度为0.6 mg/L IAA+2 mg/L 6-BA。苗龄为6 d的子叶出愈情况最好。诱导不定芽的最适植物生长调节剂浓度为0.3 mg/L IAA+2 mg/L 6-BA。在培养基中添加200、600 mg/L的活性炭或10 mg/L的VC,愈伤组织和不定芽形成过程中的褐化现象可得到有效控制。最适生根方法为两步生根法,其最佳的NAA浸泡浓度为50 mg/L。最适条件下,50 d左右便可得到完整的再生植株。

A complete set of in vitro propagation system of tomato was established and the browning phe-nomenon in this process was studied.The results showed that the optimum explant for callus inducing was cot-yledon,and the optimum plant growth regulator was 0.6 mg/L IAA＋2 mg/L 6-BA.The 6-day cotyledon had the best callus induction effect.The optimum plant growth regulator concentration for budding was 0.3 mg/L IAA＋2 mg/L 6 -BA.Adding 200,600 mg/L activated carbon or 10 mg/L VC into medium,the browning phenomenon could be controlled effectively in the formation of calluses and adventitious buds.The optimum rooting method was indirect rooting method,and its optimum NAA concentration was 50 mg/L.Un-der these optimum conditions,a whole regenerated plantlet could be obtained in about 50 days.