基于UPLC/TOF-MS的玻璃苣家兔体内移行成分分析

Analysis of the Constituents Absorbed in Rabbits after Oral Administration of Borago Officinalis Based on UPLC/TOF-MS

目的:分析玻璃苣的体内作用物质。方法:利用超高效液相色谱-飞行时间质谱联用技术(UPLC/TOF-MS)对家兔口服玻璃苣后尿液和血清中的移行成分进行分析。结果:在尿液中共发现5个玻璃苣体内成分,其中4个成分在不同时间采集的血清UPLC/TOF-MS图中离子强度较低,而其中1个成分具有较高的离子强度,初步推测为穿心莲内酯。结论:血液和尿液中成分将成为玻璃苣的体内作用物质,有利于初步阐述玻璃苣的有效成分以及作用机理。

Objective： To analyze the constituents of Borago officinalis in vivo. Methods： The urine and serum constituents in rabbits after oral administration of Borago officinalis were analyzed by using ultra performance liquid chromatography time of flight mass spectrometry（ UPLC- TOF / MS）. Results： It was found that five components were in the borage officinalis of urine,four components in different collection time of serum UPLC / TOF- MS were in lower ionic strength,and ionic strength was high in one compound,Which may be andrographolide contents by initially presumed. Conclusion： The composition of serum and urine will become the constituents in vivo,which will be conducive to explain accurately the effective components and mechanism of borage officinalis.