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α-玉米赤霉醇对大鼠成骨细胞IL-6表达及分泌的影响

Effects of α-zeranol on the IL-6 expression and secretion in rat osteoblasts
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摘要 目的：探讨α-玉米赤霉醇对体外培养的大鼠成骨细胞IL-6表达及分泌的影响。方法体外培养原代SD新生大鼠颅骨成骨细胞，形态学观察、碱性磷酸酶（ALP）染色、I型胶原免疫荧光染色进行成骨细胞鉴定，分别以雌激素及不同浓度的α-玉米赤霉醇作用于成骨细胞，采用RT-PCR法检测IL-6 mRNA的表达，ELISA法检测成骨细胞培养上清中IL-6的含量。结果10^-10-10^-5mol/Lα-玉米赤霉醇能够抑制成骨细胞IL-6 mRNA的表达及其蛋白分泌，其中以10-6 mol/L、10-7mol/L的α-玉米赤霉醇作用显著。结论α-玉米赤霉醇能够抑制成骨细胞细胞因子IL-6 mRNA及蛋白的表达，这可能是α-玉米赤霉醇防治绝经后骨质疏松症的机制之一。 Objective To investigate the effects ofα-zeranol on the IL-6 expression and secretion in rat osteoblasts. Methods Osteoblasts were isolated from the skull of SD neonatal rats and identified by morphological observation, ALP staining, and collagen I immunolfuorescence staining. 17β-estradiol andα-zeranol with different concentration were administrated. The expression of IL-6 mRNA in the osteoblasts was detected by RT-PCR and the level of IL-6 in culture supernatant of osteoblasts was measured by ELISA. Results α-zeranol with 10^-10-10^-5 mol/L concentrations could inhibit the expression and secretion of IL-6 in osteoblasts, among which the concentrations of 10-6 and 10-7 mol/L had obvious effects. Conclusionα-zeranol can inhibit the expressions of IL-6 mRNA and protein in osteoblasts, it may play an important role in the prevention of postmenopausal osteoporosis.
作者 段斐 郝华超 薛娟 李少春 刘未华 谷雨 孙晓芳
机构地区 河北大学基础医学院
出处 《医学研究与教育》 CAS 2014年第1期1-7,共7页 Medical Research and Education
基金 河北大学医学学科建设项目(2012A2002) 河北省计生委基金项目(2010A03)
关键词 Α-玉米赤霉醇 成骨细胞 白细胞介素6 绝经后骨质疏松症 IL-6 α-zeranol osteoblasts postmenopausal osteoporosis
分类号 R977 [医药卫生—药品]
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参考文献22

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二级参考文献42

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医学研究与教育
2014年 第1期

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