陈皮提取物的抗氧化活性研究

Study on Anti-oxidation Activity of Orange Peel Extract

通过乙醇浸提法对陈皮中黄酮类化合物进行提取并对其含量进行测定,进一步通过铁还原能力实验和羟自由基消除率实验测定陈皮中黄酮提取物的抗氧化活性。研究结果表明:陈皮中黄酮化合物的含量达到24.43%,铁还原能力试验表明,陈皮提取物的IC50值为1.354 mg/ml,羟自由基清除率实验表明,当陈皮黄酮提取物的浓度为4 mg/ml时,清除率达到82.56%,其IC50值为1.340mg/ml。并且黄酮类化合物的浓度的增加,其抗氧化活性逐渐增强。

The ethanol extraction were used to extract flavonoids from dried orange peel and determine the content of iron reduc-tion through further experiments and hydroxyl free radical elimination capacity determination of antioxidant activity of flavonoids ex-tracts dried orange peel test rate The results showed that： the content of flavonoids in Pericarpium Citri Reticulatae reached 24.43%ferric reducing ability test showed that the extract of Pericarpium Citri Reticulatae IC50 value is 1.354 mg/ml the rate of hydroxyl radical scavenging experiment showed that when concentration of citrus flavonoids 4 mg/ml extract too removal rate82.56%. the IC50 value is 1.340 mg/ml And increase the concentration of flavonoids antioxidant activity increased.