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宫颈癌细胞系中HER-2/neu的表达及机制的研究 被引量：1

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摘要 目的:研究HER-2/neu在宫颈癌细胞系中表达的作用,为宫颈癌的机制探讨和临床靶向治疗提供新的理论依据。方法:通过免疫细胞化学检测宫颈癌细胞系HeLa、SiHa中HER-2/neu的表达情况,将SiHa细胞转染HER-2/neu过表达载体后进行细胞计数和MTT实验。对HER-2/neu过表达细胞进行成球实验。结果:HeLa细胞高表达HER-2/neu,SiHa细胞低表达Her-2/neu。将SiHa细胞中的HER-2/neu过表达后细胞增殖加快且活性增强,同时成球能力增强。结论:不同的宫颈癌细胞系HER-2/neu蛋白表达水平不同,HeLa细胞高表达而SiHa细胞低表达。宫颈癌细胞系SiHa细胞过度表达HER-2/neu,可以促进细胞的增殖活性和自我更新,提示HER-2/neu可加速宫颈癌细胞的侵袭性。
作者 李连香 李玢 陈丽宏
机构地区 陕西省人民医院
出处 《内蒙古中医药》 2014年第2期33-34,共2页 Inner Mongolia Journal of Traditional Chinese Medicine
基金 陕西省社发攻关资助项目<MicroRNAs与宫颈癌发生发展及预后的关系研究>资助 项目编号(2010K01-156)
关键词 HER-2 宫颈癌细胞 肿瘤增殖 肿瘤自我更新
分类号 R737.33 [医药卫生—肿瘤]
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参考文献10

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二级参考文献18

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共引文献6

同被引文献17

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内蒙古中医药
2014年 第2期

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