大鼠髁突软骨增殖细胞核抗原表达的昼夜节律

Circadian rhythm of the expression of proliferating cell nuclear antigen in condylar cartilage of growing rats

目的 :了解髁突软骨细胞增殖的昼夜节律性 ,为探讨一天中临床功能矫形治疗介入的最佳时段提供初步实验依据。方法 :应用免疫组织化学方法对生长期大鼠髁突软骨细胞增殖细胞核抗原 (PCNA)表达进行检测分析。结果 :大鼠髁突软骨细胞的 PCNA表达 ,存在明显的昼夜节律性 ;其峰值相位约在12 :0 0 ,谷值相位约在 0 :0 0。结论 :髁突软骨细胞增殖存在昼夜差异性 ,其高峰期在白天。功能矫治一天中 。

砄bjective: To study the circadian rhythm of cell proliferation in condylar cartilage of growing rats.Methods:Samples of condylar cartilage of 4 week SD rats were obtained at 0∶00,4∶00,8∶00,12∶00,16∶00 and 20∶00 respectively. The expression of proliferating cell nuclear antigen (PCNA) in the samples was studied with immunohistochemical method.Results:The expression (% of positive cells) of PCNA in the condylar cartilage showed a significant circadian rhythm.The acrophase and bathyphase of the expression were at 12∶00 (3.06±0.54) and 0∶00 (1.67±0.47) respectively.Conclusion:The condylar growth in growing rats is not even during a day and night,the most active growth takes place in day time.