口腔鳞癌nm23-H1基因存在状态和颈淋巴结转移的关系

Expression of nm23-H1 gene in oral squamous cell carcinoma and its relationship with metastasis to neck lymph nodes

目的 :探讨口腔鳞癌中 nm2 3- H 1基因结构变化和表达水平及其和颈淋巴结转移的关系。方法 :用PCR法扩增了 30例人口腔鳞癌 ,7例正常口腔粘膜标本及人舌鳞癌细胞系 TSCCa和人口腔转移癌细胞系GNM nm2 3- H 1基因 ,并对 PCR产物进行 SSCP分析 ;用原位杂交法检测鳞癌组织及两细胞株 nm2 3- H 1m RNA水平 ,对其和颈淋巴结转移的关系进行统计学分析。结果 :除 1例口腔鳞癌外 ,所有标本均无 nm2 3-H 1结构变化 ;9例有转移的口腔鳞癌组织中有 7例 nm2 3- H 1m RN A表达阴性 ,而 2 1例无转移患者只有 5例表达阴性 ,且 GNM nm2 3- H 1阳性率低于 TSCCa(4 1.2 %对 6 3.1% ) ,nm2 3- H 1表达与癌转移呈显著负相关 (P<0 .0 1) ;nm2 3- H 1表达和组织学分级无关。结论 :nm2 3- H 1m RNA水平的变化不是由基因结构变化导致 ;nm2 3- H

砄bjective:To investigate the variation of nm23 H1 gene structure and its expression in oral squamous cell carcinoma (OSCC) and to study the relationship between nm23 H1 expression and OSCC metastasis to neck lymph nodes.Methods: nm23 H1 gene in the samples of 30 cases of OSCC,7 of normal oral mucous tissue,human OSCC cell line TSCCa and human oral metastatic cancer cell line GNM was extracted and amplified by PCR. PCR products were investegated with SSCP analysis; nm23 m RNA level in the samples was studied by in situ hybridization.The relationship between mRNA expression and metastasis to neck lymph nodes was analyzed statistically.Results: All samples showed no change in nm23 H1 gene structure except deletion in one case of OSCC;7 out of 9 cases with metastasis to neck lymph nodes showed no hybridization signal of nm23 H1 mRNA,however,only 5 were found no nm23 H1 mRNA in 21 cases without metastasis,positive rate of nm23 H1 expression in GNM cells was lower than that in TSCCa (41.2% versus 63.1%);The expression of nm23 H1 gene showed significant negative relationship with neck lymph nodes metastasis ( P <0.01);no relationship was found between expression of nm23 H1 gene and cancer pathological classification.Conclusion:The change of nm23 H1 expression doesn't result from the variation of gene structure;the product of nm23 H1 gene probably inhibit OSCC metastasis to neck lymph nodes.