钛网-珊瑚复合支架的组织工程骨研究

Fabrication of tissue engineered bone by seeding osteoblasts into titanium coral scaffolds

目的 :建造用钛网增强的组织工程骨 ,使其在结构、力学特性上更接近正常骨组织 ,以满足承力部位植骨的需要。方法 :将钛网制备成直径 8mm,长 12 mm的圆柱状 ,填入直径 2～ 3mm的珊瑚颗粒 ,成为复合支架材料。在复合支架中植入骨髓来源的成骨细胞 ,体外继续培养 2 d后 ,植入裸鼠背部皮下组织中 ,2个月后取材。通过大体标本观察、X线片检查、磨片的组织学检查 ,观察支架中新骨的形成情况及与钛网的愈合情况。结果 :大体标本观察 ,新形成的组织为均匀的暗红色 ,表面光滑 ,钛网位于其中 ;X线片检查表明复合支架中有大量 X线阻射影 ,均匀一致 ,与钛网间无间隙 ;磨片的组织学检查证实 ,在支架中有大量成熟的新骨形成 ,并与钛网愈合良好。结论 :钛网可与组织工程骨相互愈合 ,用于承力部位植骨 。

砄bjective:To fabricate bone tissue that has similar structural and mechanical characters with normal bone.Methods: Titanium meshes were molded into the shape of column in the length of 12 mm and in the diameter of 8 mm. The column was filled with natural coral granduls.4×10 7 marrow derived osteoblasts in 200 μl cell culture medium were seeded into each of five scaffolds and incubated in vitro for 2 d to ensure that cells adhere well on the scaffolds. Then the scaffolds were implanted subcutaneously into the back of nude mice. Two months after implantation, the animals were sacrificed and the implanted materials were investigated by gross specimen inspection, X ray examination and histological observation. Results:2 months after in vivo incubation, the newly formed tissue was red and had the gross appearance of bone, and kept the original shape of column. Titanium mesh situated in the surface area. X ray examination showed that large amount of new bone formed in the scaffolds, there was no space between new bone and titanium mesh. Most of coral granduls had been absorbed. Histological observation demonstrated that in the surface area, new bone integrated well with titanium mesh and was enforced by titanium mesh(like cortical bone), and in the middle area large amount of lamellar bone formed.Conclusion: Newly formed bone in this experiment has similar structural with normal cortical bone.