温固化水凝胶的制备和应用

Preparation and application of temperature dependent hydrogel

目的 制备出适合于组织工程用的温固化型水凝胶 ,并将其应用于可注射软骨的建造 ,观察实验结果 .方法 制备不同浓度的水凝胶 ,并添加不同的物质 ,测量其凝胶化温度 ,并把水凝胶用于裸鼠组织工程软骨的建造 .注射后 4,8和 12 wk取材 ,通过大体标本和组织学观察研究软骨的形成情况 .结果 2 5 0 g· L- 1的水凝胶具有恰当的凝胶化温度和能满足要求的力学性能 .含钙磷的添加物因溶解度不高 ,对凝胶的性能未能产生显著影响 .此水凝胶用于软骨组织工程 ,注射后 4wk在皮下形成稍硬的结节 .8wk后形成的结节质地坚韧 ,切面色泽亮白 .组织学观察 4wk即有软骨形成 ,但不成熟 ,8wk标本有成熟软骨形成 ,12 wk标本成熟软骨的量进一步增加 .结论 温固化型水凝胶凝胶化温度受浓度影响 ,2 5 0 g· L- 1 水凝胶凝胶化温度为 5℃ ,可用于组织工程软骨的建造 。

AIM To prepare temperature dependent hydrogel and to apply it as the scaffold in fabrication of tissue engineering cartilage and to observe the results.Various concentration and additives of hydrogel were prepared, the gelation temperature were determined, the most favorite hydrogel (250 g·L -1 ) was selected to be used as the scaffold of tissue engineering, and then the cartilage was fabricated. The chondrongenesis was studied histologically for 12 weeks. RESULTS Gelation temperature of 250 g·L -1 hydrogel was 5℃, and its mechanical properties were good. However the solubility of additives was too low. The 250 g·L -1 hydrogel was applied in tissue engineering; 4 weeks after injection, the hard nubble was formed subcutaneously and immatural cartilage was observed. 8 weeks after injection matural catilage was observed. 12 weeks after injection the amount of matural cartilage increased. CONCLUSION Gelation temperature depends on the concentration of hydgogel, the gelation temperature of 250 g·L -1 hydrogel being 5 ℃. The hydrogel could be applied in fabrication of tissue engineering cartilage, and the cartilage could be fabricated successfully.