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DPPH法测定芒果叶中总黄酮提取物的抗氧化活性 被引量:12

Determination of Antioxidant Activity of Total Flavonoids Extract in Mango Leaves with DPPH Assay in Vitro
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摘要 目的研究芒果叶中不同含量总黄酮提取物的抗氧化活性。方法采用DPPH法测定芒果叶中以芒果苷为指标的不同含量的总黄酮提取物的抗氧化活性。结果抗氧化活性由大到小依次为:VC>芒果苷含量为78.48%的总黄酮提取物>芒果苷对照品>芒果苷含量为52.13%的总黄酮提取物>芒果苷含量为38.65%的总黄酮提取物>芒果苷含量为15.24%的总黄酮提取物>BHT。结论不同含量的总黄酮提取物对DPPH自由基的清除作用呈现一定的线性关系。 Objective To investigate the in-vitro anioxidant activity of total favonoids extract with different percent- ages of mangiferin content from mango leaves. Methods DPPH assay was applied for the determination of antioxidant activity of total flavonoids extract from mango leaves with different contents of mangiferin. Results The antioxidant ac- tivity of the total flavonoids extracts was in decreasing sequence, and they were VC, total flavonoids extract with 78.48 % of mangiferin, mangferin control, total flavonoids extract with 52.13 % of mangiferin, total flavonoids extract with 38.65 % of mangiferin, total flavonoids extract with 15.24 % of mangiferin, and BHT. Conclusion The scavenging effect of total flavonoids extract on DPPH free radicals presents a linear relationship with the percentages of mangferin content.
出处 《中药新药与临床药理》 CAS CSCD 北大核心 2014年第2期185-188,共4页 Traditional Chinese Drug Research and Clinical Pharmacology
基金 江西创新药物与高效节能降耗制药设备协同创新中心[赣教高字(2012)89号]
关键词 芒果叶 总黄酮提取物 芒果苷 DPPH 抗氧化活性 Mango leaves Total flavonoids extract Mangiferin DPPH Antioxidant activity
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