前列地尔与川芎嗪、黄芪合用对大鼠心肌缺血再灌注损伤的保护作用

Protective effect of alprostadil combined with ligustrazine and Astragalus membranaceus on myocardial ischemia reperfusion injury in rats

目的 :观察前列地尔与川芎嗪 (LT )、黄芪(AM ) 3药合用对大鼠心肌缺血再灌注损伤的保护作用。方法 :采用在体大鼠开胸结扎冠状动脉左室支 30min后 ,松扎再灌注 60min造成心肌缺血再灌注模型并以 0 .9%氯化钠注射液为模型对照 ,观察 3药 :前列地尔 31.2 5μg·kg- 1,川芎嗪 2 5mg·kg- 1,黄芪 4 15mg·kg- 1合用对再灌注心肌组织中超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶(GSH PX)活力 ,丙二醛 (MAD)、Ca2 +含量及血清肌酸磷酸激酶同功酶 (CK MB)含量的变化 ,并同时心电图监测心律失常情况。结果 :与模型对照组相比3药合用可提高再灌注心肌组织中SOD ,GSH PX活力 ,差异有非常显著意义 (P <0 .0 1) ;并能降低再灌注心肌组织中MDA ,Ca2 +含量及血清CK MB含量 ,差异有非常显著意义 (P <0 .0 1) ;防止再灌注室性心律失常的发生 ,缩短心律失常的维持时间 ,降低ST段抬高的程度 ,差异有非常显著意义 (P <0 .0 1)。结论 :前列地尔与LT ,AM合用在保护心肌缺血再灌注损伤中有显著的作用 。

AIM: To study the protective effect of alprostadil combined with ligustrazine (LT) and Astragalus membranaceus (AM) on myocardial ischemia reperfusion injury in rats. METHODS: The myocardial ischemia reperfusion model was induced by 30 min coronary occlusion and 60 min reperfusion in openchest anesthetized rats. The 0.9% sodium chloride injection was as a model control. The superoxide dismutase (SOD), glutathione peroxidase (GSH P X) activities, malondialdehyde (MDA) and Ca 2+ contents in myocardium, serum creatine kinase MB (CK MB) were determined. in the course of reatment with Aiprostadil combined with astragalus membranaceus and LT arrhythmia was monitroed with electrocardiogram lead Ⅱ. RESULTS: Alprostadil (31.25 μg·kg -1 ) combined with AM(415 mg·kg -1 ) and LT (25 mg·kg -1 ) effectively antagonized the reperfusion injury. It prevented the incidence of reperfusion ventricular arrhythmias (VT and VF), retarded the appearing time and shortened the duration of arrhythmias, lowered the elevated ST segment as well. It decreased significantly the myocardial Ca 2+ , MDA contents and CK MB release as well as increased the myocardial SOD and GSH P X activities in comparison with the model control ( P <0.01). CONCLUSION: Alprostadil combined with AM and LT has protective effect on myocardial ischemia reperfusion injury when they are used in combination. The protective effect may be related to scavenging the oxygen free radicals formed during reperfusion.