抗菌肽KS26基因的串联及在毕赤酵母中的表达

The Tandem of Antibacterial Peptide KS26 Gene and Expression in Pichia Pastoris

构建小肽基因的串联体,对其在真核系统中表达、纯化和性质研究具有重要的意义.本研究利用SOE-PCR的方法获取小分子多肽的全基因,利用同尾酶将基因串联,并将其插入到PICZα真核表达载体中诱导表达.经酶切和DNA序列分析表明,该串联体基因与所设计的基因序列完全一致;SDS-PAGE的分析结果显示,该小肽基因在毕赤酵母中有较高水平的表达.

It is very important to tandem construction, expression, purification, characteristic research of small peptide gene. In this study, the whole-gene sequence of antibacterial peptide was achieved by means of SOE- PCR, constructed gene tandem through isocaudarner method, and constructed recombinant eukaryotic expression vector. Enzyme digest and sequencing showed that gene tandem was equal to the sequence of the designed gene. SDS-PAGE analysis indicated that antibacterial peptide gene expressed highly in pichia pastoris.