维药罗勒子油脂的定性定量研究

The research of qualitative and quantitative of oil Uighur medicine of Seme Ocimi Basilici

目的建立罗勒子中亚油酸薄层鉴别及气相色谱法测定罗勒子油脂中各脂肪酸含量的方法。方法以石油醚-乙酸乙酯(37∶3)为展开剂,薄层色谱法鉴别罗勒子中亚油酸。乙醚提取罗勒子油脂,气相色谱法测定罗勒子油脂中各脂肪酸的含量。结果薄层鉴别的Rf值较合适,紫外灯检视,供试品色谱中,在与对照品色谱相应的位置上,显相同的蓝色荧光斑点。罗勒子油脂中各种脂肪酸在50min内完全分离,测得各脂肪酸的相对含量分别为肉豆蔻酸(23.34%)、软脂酸(15.65%)、γ-亚麻酸(32.23%)、亚油酸(28.34%)、油酸(19.35%)及花生四烯酸(14.69%)。结论本法操作简便,结果准确可靠,重复性好,可为罗勒子的质量标准提供参考数据。

Objective To establish the methods of TLC identification of linoleic acid and to determine the contents of every fatty acid in Seine Ocimi Basilici oil by GC. Methods The petroleum ether and ethyl acetate （37：3） as developing agent. The basil of linoleic acid was identified by TLC method. The oil was extracted by ether, and the contents of every fatty acid in Seine Ocimi Basilici oil were determined by GC. Results The Rf value is more appropriate, it showed the same blue fluorescence spots with the standard chromatography corresponding position in the sample chromatography by UV lamp review. The Seine Ocimi Basilici oil was composed of myristic acid （23.34%）, palmitic acid （15.65%）, oleic acid （32.23%）, linoleic acid （28.34%）, linolenic acid （19.35%）, arachidonic acid （14.69%）, and the separation of all kinds of fatty acids was completed within 50 minutes. Conclusion The method of operation was simple, results were accurate and reliable, and had a good repeatability. It can provide reference data for the quality standards of Seme Ocimi Basilici.