大鼠局灶性脑缺血与缺血再灌注损伤IL-8的研究

The Change of the Level of Interleukin 8 in Focal Cerebral Ischemia and Ischemia reperfusion Injury in Rats

目的 探讨IL 8在脑缺血损伤及缺血再灌注损伤中的作用。方法 (1)采用改良ZeaLonga线栓法大鼠大脑中动脉闭塞 (middlecerebralarteryocclusion ,MCAO)模型。 (2 )应用双抗体夹心间接ELISA法检测脑缺血组与缺血再灌注组大鼠受损脑组织和血清中IL 8的浓度。结果 (1)脑缺血再灌注组受损脑组织中IL 8含量比脑缺血组高 (P <0 . 0 5 ) ,二者IL 8含量的变化均呈时间依赖性。前者于再灌注 2 2h达高峰之后很快下降 ;后者于缺血 6h达高峰 ,之后缓慢下降。(2 )脑缺血再灌注组血清IL 8浓度于再灌注 1h达峰值(7.0 8± 1.36 ) pg/mL ,之后很快降至较低水平 ;而在脑缺血组 3h最高 ,为 (3.6 1± 0 .81) pg/mL ,随后缓慢下降。结论 脑缺血和脑缺血再灌注损伤均有IL 8参与 ,IL 8在脑缺血再灌注损伤中所起的作用较在脑缺血损伤中大。

Objective To study and compare the role of interleukin 8(IL 8) in cerebral ischemia injury and cerebral ischemia reperfusion injury. Methods The middle cerebral artery(MCA) of rats were occluded by the modified Zea Longa′s technique for different time or followed by reperfusion for different time.IL 8 was detected with double antibody sandwich indirect ELISA of injured brain tissue and blood sera of the two groups. Results The content of IL 8 in injured brain tissue in cerebral ischemia reperfusion group was higher than that in cerebral ischemia group.They both changed time dependently.The former peaked at 22 hours after reperfusion,and then decreased rapidly.The latter peaked at 6 hours after ischemia,and then decreased slowly.The concentration of IL 8 in serum peaked at 1 hour after reperfusion, and decreased rapidly in ischemia reperfusion group.While in ischemia group, it peaked at 3 hours after ischmia,and then decreased slowly. Conclusions IL 8 is involved in both ischemia reperfusion injury and ischemia injury. The damage caused by IL 8 in cerebral ischemia reperfusion injury is more severe than in cerebral ischemia injury.