肌型肌酸激酶同工酶亚型在早期诊断假肥大型肌营养不良中的价值

The Uses of Isoforms of Creatine Kinase CK MM in the Early Diagnosis of the Patient with Duchenne Muscular Dystrophy

目的 研究假肥大型肌营养不良 (DMD)患者肌型肌酸激酶 (CK MM )亚型的变化 ,为早期诊断和正确评价病情提供依据。方法 采用不连续缓冲体系 ,在稳流低压条件下电泳分离CK MM亚型 ,荧光扫描。结果 随着DMD患者病情的加重 ,其不同阶段的MM2 /MM1均与对照组差异显著 (P <0 .0 5 ) ;DMD患者不同阶段的MM2 /MM1值差异也显著 (P <0 .0 5 )。结论 CK MM亚型的改变是DMD的早期诊断指标 ,是判断病情及科学评价治疗效果的依据。

Objective To study the changes of isoforms of creatine kinase CK MM in patients with Duchenne muscular dystrophy for the purpose of early diagnosis as well as evaluation of seriousness of the disease. Methods A dis continuous buffer system was used. CK MM isoform was separated by electrophoresis under the condition of constant current and low voltage, then fluorescence scanning. Results The nature of DMD patient was gradually getting worse along with the age increase. There was a statistically significant change of the isoform ratio MM 2/MM 1 in each stage of DMD compared with the normal control ( P< 0.05 ); there was also significant change of the isoform ratio MM 2/MM 1 between each stage of the subject ( P < 0.05 ). Conclusions The change of CK MM isoforms is a specific indicator in early diagnosis of DMD. It is a important indicator for evaluation of seriousness of the disease. It is also useful in the evaluation of the real effect of some treatment methods