摘要
目的 :观察 IL - 1β对基础状态及精氨酸加压素 (AVP)诱导心肌成纤维细胞 (CFs)增殖和 p2 7蛋白表达的影响。方法 :采用胰酶消化 ,差速贴壁法培养 CFs,四氮唑盐 (MTT)比色法检测细胞增殖 ,碘化丙啶 (PI)标记细胞DNA,间接免疫组化染色标记细胞内的 p2 7蛋白 ,用流式细胞分析仪 (FCM)技术测定细胞周期和 p2 7蛋白表达的阳性率。结果 :11× 10 - 7mol/ L AVP组 MTT法测定的 CFs的吸收 (A)值 (0 .386± 0 .0 11)较基础状态 (0 .32 4± 0 .0 0 7)明显升高 (P<0 .0 1) ;1× 10 5 U/ L IL - 1β单独作用组 (0 .2 98± 0 .0 30 )和 AVP+IL - 1β组 (0 .332± 0 .0 41)的 A值均分别较基础状态和 AVP组显著降低 (均 P<0 .0 1) ;2 IL- 1β组和 AVP+IL- 1β组 CFs的 S期细胞百分率和细胞增殖指数 (PI)分别较基础状态组和 AVP组明显降低 ,而 G0 / G1 期细胞百分率则分别高于上述两组 (均 P<0 .0 1) ;3IL- 1β组和 AVP+IL- 1β组 CFs的 p2 7蛋白表达阳性率分别为 (95 .0 3± 1.0 2 ) % ,(88.2 3± 2 .87) % ,分别高于基础状态 (78.45± 1.91) %和 AVP组 (6 3.30± 1.85 ) % ,并且有统计学意义 (均 P<0 .0 1)。结论 :IL - 1β通过升高细胞内 p2 7蛋白表达水平参与 CFs增殖的负调控过程。
AIM:To study the effect of IL 1β on cardiac fibroblasts(CFs) proliferation and p27 expression under basal condition and induced by arginine vasopressin (AVP). METHODS: Neonatal rat CFs was cultured with enzymatic dissociation . MTT assay was used to measure cell prolifeation.Labled with PI,FITC,respectively,DNA labled with PI and p27 expression were determined through flow cytometre (FCM) assay. RESULTS: ①A value of CFs measured with MTT assay in basal condition and AVP group were 0.324±0.007 and 0.386±0.011,respectively,which were significantly higher than that in IL 1β group and AVP+IL 1β group. ②Percentage of S stage cell and proliferation index (PI) in IL 1β group and AVP+IL 1β group were significantly lower than those in basal and AVP group, respectively, with commitment of an apparent increase of G0/G1 stage percent (both P <0.01). ③Positive rate of p27 expression in IL 1β group was (95.03±1.02)%,which was higher than that of basal condition group(78.45±1.19)%( P <0 01).Moreover, positive rate of p27 in AVP+IL 1β group (88.23±2.87)% was also higher than that in AVP group(63.30±1.85)%( P <0.01). CONCLUSION :IL 1β play an important negative role in CFs proliferation by upregulating p27 expression.
出处
《心脏杂志》
CAS
2001年第1期8-10,共3页
Chinese Heart Journal
