摘要
目的研究凌霄花提取物抗凝血作用,确定凌霄花抗凝血活性部位。方法通过血浆复钙实验测定小鼠血浆复钙时间(PRT)和断尾法测定小鼠出血时间(BT)实验,比较凌霄花不同提取物的药效活性。结果凌霄花水提物、醇提物及水提醇沉上清液均具有抗凝血活性;醇提物萃取后正丁醇层药效最显著;水提醇沉上清液过大孔树脂后10%乙醇部位药效最显著。结论凌霄花抗凝血活性主要存在于极性较大部位。
Objective To investigate the anti-coagulation of the extracts from the flowers of Campsis gradilora （CG） and to determinethe active fraction. Methods Plasma recalcification time （PRT） of mice was evaluated in plasma recalcification experiment and bleeding time （BT） was determined by cutting-tail method. The anti-coagulation activities of different fractions in the extracts from CG were compared. Results The water extract, 80% ethanol extract, and the extract by water-extraction and alcohol-precipitation from the flowers of CG all have the anti-coagulation activities. The butanol layer from 80% ethanol extract and the macroporous resin eluate of 10% ethanol from the extract by water-extraction and alcohol-precipitation showed the significant anti-coagulation. Conclusion The more polar fractions of the extracts from the flowers of CG are the main effective fractions
出处
《药物评价研究》
CAS
2014年第1期17-20,共4页
Drug Evaluation Research
基金
上海市卫生局中医药科研基金资助项目(WSJ1219)
关键词
凌霄花
有效部位
血浆复钙时间
出血时间
黄酮
Campsis gradiflora （Thunb.） K. Schum.
active fraction
plasma recalcification time
bleeding time
flavone
