3种免疫增强剂对黄颡鱼非特异性免疫机能的影响

Effect of Three Kinds of Immunostimulants on the Non-specific Immunity Functions of Pelteobagrus fulvidraco

[目的]研究酵母细胞壁、黄芪多糖、芪参散这3种免疫增强剂对黄颡鱼非特异性免疫功能的影响,为其在水产养殖中的推广和应用提供参考。[方法]制备含有不同剂量酵母细胞壁、黄芪多糖、芪参散药饵连续投喂42天,并在试验的0、7、14、28天检测黄颡鱼血清的非特异性免疫指标,分析其对黄颡鱼免疫功能的影响。[结果]酵母细胞壁、黄芪多糖、芪参散这3种免疫增强剂对黄颡鱼血清补体C3、SOD、溶菌酶、血清白蛋白均有不同程度提高。免疫攻毒试验表明3种免疫增强剂均有一定的免疫保护作用,其大小次序为:酵母细胞壁组>黄芪多糖组>芪参散组,其中2和4 g/kg的黄芪多糖和酵母细胞壁组保护率最高,为50%。[结论]饲料中添加酵母细胞壁、黄芪多糖和芪参散均对黄颡鱼的非特异性免疫功能具有一定的促进作用。

[ Objective ] The present research aimed to provide reference for the popularization and application of the three kinds of lmmunos- timulants in the aquatic feeds. [ Method] Different dose of Astragalus polysaccharide, yeast cell wall and Ginseng powder were added in the feed of Pelteobagrus fulvidraco to analyze their effects on the non-specific immunity function of P. fulvidraco. [ Result] Compared with control group,, Astragalus polysaccharide, yeast cell wall and Ginseng powder could increase lysozyme activity in serum, serum albumin, SOD activity in serum and complement C3 content in serum. The three kinds of Immunostimulants could provide a good protection against the infection of Edwardsiella cctaluri. The immunity protection rate against E. cctaluri was 50% when P. fulvidraco was fed with 2 g/kg Astragalus polysac- charide or 4 g/kg yeast cell wall. [ Conclusion] Adding Astragalus polysaccharide, yeast cell wall and Ginseng powder in the feed had certain promotion on the non-specific immunity function of P. fulvidraco.