肠膜明串珠菌6055生产低聚葡萄糖的研究

Research of the glucooligosaccharides as prebiotics produced by Leuconostoc mesemteroide 6055

对肠膜明串珠菌6055代谢生成低聚葡萄糖的适宜发酵条件研究表明,菌株6055的最适发酵培养基为:蔗糖100g/L、麦芽糖50g/L、酵母膏5g/L、磷酸氢二钾3g/L、吐温80 2mL;在此基础上,采用接种量为3.0%,发酵温度25.0℃,pH为7.10的培养条件时,菌株6055生成的低聚葡萄糖量最多,为5.15g/L。在发酵液中接入2%的酿酒酵母,发酵28h后,可除去发酵液中100%的果糖和葡萄糖以及92.8%的麦芽糖,使低聚葡萄糖占总糖的比例由37.5%升高至74.3%,以此建立了酿酒酵母除糖的低聚葡萄糖纯化工艺。对来自菌株6055产生的低聚葡萄糖评价表明,2%的这种低聚葡萄糖可促进嗜酸乳杆菌NCFM和双歧杆菌Bb-02分别增殖37.9%和48.6%,具有明显的促进肠道有益微生物生长的效果。

Single factor experiment, orthogonal test and response surface analysis were used to determine the optimal medium components and fermentation condition available for L.mesenteroides 6055 to synthesize glucooligosaccharides.The results showed that the optimum media favoring glucooligosaccharides formation were： sucrose（ 100g/L）, maltose （ 50g/L）, yeast extract （ 5g/L）, K2 HPO4 （ 3g/L）, and Tween 80 （2mL） .The optimum fermentation condition for L.mesenteroides 6055 was inoculum of 3.0%, 25.0℃, and pH7.10, and the yields of glucooligosaccharides reached 5.15g/L.Addition of 2.% Saccharomyces cerevisiae to fermentation liquor is able to metabolize selectively 100% fructose and glucose, and 92.8% maltose, the proportion of glucooligosaccharides formed by L.mesenteroides 6055 increased from 37.5% to 74.3%.And the glucooligosaccharides produced by L.mesenteroides 0055 whose concentration is 2% promoted proliferation of gastro-intestinal probiotic strains such as Bifidobacterium animalis subsp.lactics Bb-02 and Lactobacillus acidophflus NCFM by 37.9% and 48.6%, respectively.