响应曲面法优化麒麟菜提胶残渣中膳食纤维的提取工艺

Optimized extraction process of soluble dietary fibre from the Eucheuma carrageenan residue by response surface methodology

以麒麟菜提胶残渣为原料,以提取温度、时间、液料比为实验因素,可溶性膳食纤维(SDF)得率为响应值。分别通过单因素及Box-Behnken响应曲面实验优化SDF提取工艺,并通过响应曲面实验分析提取因素对SDF得率的影响。结果表明,三个提取因素的不同水平对得率有显著的(p<0.05)影响,提取温度与液料比和提取时间对得率有显著的(p<0.01)交互作用,并且临界值之间呈线性负相关;以SDF得率最大值为响应值,得到最优提取工艺为:提取时间3.2h、提取温度90℃、液料比85mL/g;此条件下所得的SDF得率为5.035%±0.036%。可以为麒麟菜提胶残渣的再利用提供可行指导。

The aim was to study the influence of 3 factors including extracting temperature, extracting time and liquid -solid ratio on the yield of SDF from Eucheuma carrageenan residue by response surface methodology.On the basis of single-factor investigation and Box-Behnken central composite experiments,the processing technology of SDF extration was optimized, and the extracting factors effection on yield of SDF was analyzed by response surface experiments.The results showed that the extrapn rate was significant effected （ p 〈 0.05） by different levels of 3 factors and there was significant interaction influence（ p 〈 0.01 ）on extration rate between extracting temperature and liquid-solid ratio and extracting time,and a negative linear correlation was found between critical values.Taking the maximum rate of SDF extraction as response value, the optimizing process parameters were determined to be： extracting temperature 90℃ extracting time 3.2h, liquid-solid ratio 85mL/g.Under the optimal condition ,the extraction yield of SDF from residue was 5.035% ±0.036%.The processing technology could provide feasible guidance for the recycle of Eucheuma carrageenan residue.