花椒精非挥发性成分对大鼠肠道健康的影响

Effect of the non-volatile parts of Zanthoxylum essential oil on intestinal health in rats

目的:研究花椒精中非挥发性组成成分对大鼠肠道健康的影响。方法:4组实验鼠分别被灌胃相同量的大豆油、花椒精去除挥发油成分(RE)、花椒麻味物质(NTC)和花椒精除去以上两部分后的剩余物(REN)的大豆油溶液,4周后处死取样,测定各相关指标。结果:灌胃NTC组盲肠壁面积减小,游离氨浓度显著升高,短链脂肪酸含量无显著变化,盲肠内容物中乳酸菌等有益菌数量减少,大肠杆菌等有害菌数量显著上升;RE组各指标变化趋势与NTC组相同,但没有NTC作用效果显著;灌胃REN组短链脂肪酸浓度显著增大,游离氨浓度显著升高,pH呈下降趋势,盲肠壁面积增大,盲肠内容物中部分有益菌和有害菌数量均有上升趋势。结论:RE和NTC均会抑制大鼠肠道发酵,对肠道健康有害,且NTC的作用强于RE组分。

Objective：To study the effect of each components of the non-volatile parts in zanthoxylum essential oil on intestinal health in rats.Methods= Using soybean oil as blank control, the volatile removed constituents of zanthoxylum essential oil （RE）, numb taste compoents （NTC）and the volatile and numb taste components removed part of zanthoxylum essential oil （REN）which were respectivelly dissvoled into soybean oil were intragastriced other three rats groups.They were sacrificed at 28th day to assay several relevant indicators.Results： To the rats in NTC group,their cecal wall area was significantly reduced ,the free ammonia were increased, but the SCFAs didn＇t change significantly, harmful bacteria were increased with beneficial bacteria reduced.Results in RE group kept the same change trend with the NTC group in weaker effects.After intragastric,the SCFAs, cecal wall area, free ammonia and beneficial bacteria of NTC group are increased, with pH and harmful bacteria reduced.Conclusion =RE and NTC will restrain rat intestinal fermentation, have negative effect on intestinal health, and the effect of NTC is stronger than RE＇ s.