荷叶总黄酮对小鼠酒精肝损伤的保护作用

Protective effects of total flavonoids of lotus leaf( Nelumbo nuciferea Gaertn.) on alcohol-induced liver injury in mice

研究荷叶总黄酮(total flavonoids of lotus leaf(Nelumbo nuciferea Gaertn.),TFL)对小鼠酒精肝损伤的影响及作用机理。将72只小鼠随机分为正常对照组、模型组、益肝灵组、TFL低剂量组、TFL中剂量组、TFL高剂量组。除了空白组,用TFL(100、300、500mg/kg)灌胃小鼠,益肝灵组灌胃益肝灵,每日灌胃后4h用50%乙醇12mL/kg·bw灌胃,连续14d,末次乙醇灌胃6h后,处死小鼠,计算脏器指数,检测各实验组小鼠血清丙氨酸转氨酶(ALT)、天门冬氨酸转氨酶(AST)活性、甘油三酯(TG)含量、肝组织匀浆中丙二醛(MDA)、谷胱甘肽(GSH)含量、超氧化物歧化酶(SOD)、谷光甘肽过氧化物酶(GSH-Px)活性;酶联免疫法测定肝匀浆中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)的含量;对肝进行病理组织学检查。结果表明:荷叶总黄酮能显著降低酒精肝损伤小鼠血清ALT、AST、TG水平,降低肝匀浆MDA含量,抑制SOD、GSH-Px、GSH水平的下降,抑制TNF-α和IL-1β的表达,并减轻酒精导致的肝组织病理损伤,呈现剂量效应关系。说明荷叶总黄酮对酒精致小鼠肝损伤具有较好的保护作用,其机制可能与其清除自由基、抑制脂质过氧化、抑制TNF-α、IL-1β因子的表达有关。

The present study aimed at evaluating the hepatoprotective effects of total flavonoids of lotus leaf （Nelumbo nuciferea Gaertn..）（TFL）on the injury induced by alcohol in mice.Seventy-two male Kunming mice were randomly divided into control group, model group, Yiganling group and TFL group （100,300,500mg/kg）, 12 in each group.Mice were administered orally with 50% alcohol 12mL/kg at 4h after the doses of TFL everyday for fourteen consecutive days except mice in normal group.The protective effect was evaluated by biochemical parameters including serum aspartate transaminase （ AST）, alanine transferase （ ALT）, triglyceride （ TG ） and reduced glutathione （ GSH）, malondialdehyde （ MDA）, superoxide dismutase （SOD） and glutathione peroxidase （GSH- Px） in liver tissue.The protective effects on alcohol- induced liver injury of TFL were confirmed by hematoxylin-eosin staining and liver homogenate TNF-α, IL-1βcontents in liver by ELISA analysis.The results showed：Compared with model group, TFL could significantly reduce serum ALT, AST, TG levels of alcoholic- induced liver injury in mice,lower MDA content in liver homogenates, and inhibit the decrease of SOD, GSH-Px, GSH levels in a dose-dependent manner, and inhibit the expression of TNF-a and IL-lJ3 in liver tissue, and alleviate liver tissue pathological damage.This study shewed that total flavonoids of lotus leaf（ TFL） had remarkable protective effect on alcohol- induced hepatic injury in mice, possibly due to its capability of scavenging reactive oxygen species,inhibiting lipid peroxidation and expression of TNF-a and I L-lJ3cytokines.