高压静电场对荞麦幼苗抗旱能力后效性的影响

Effect of High Voltage Electrostatic Field on Drought Resistance After-effect of Buckwheat Seedlings

为了阐明高压静电场处理对荞麦幼苗抗旱能力的影响以及后效性,用电场强度0、3.0、4.0、5.0、6.0kV/cm分别处理荞麦种子15min后置于光照培养箱培养,待幼苗二叶一心期,用不同含量PEG-6000对根系干旱胁迫48h,测定叶片脯氨酸、丙二醛、可溶性糖的含量。结果表明:4.0kV/cm×15min、5.0kV/cm×15min处理条件下能够增加幼苗脯氨酸和可溶性糖含量,同时降低丙二醛含量,增强了幼苗的抗旱能力。电场生物学效应具有一定的后效性,不同电场处理下的荞麦种子其幼苗抗旱能力不同,适宜的电场处理条件是4.0kV/cm×15min、5.0kV/cm×15min。

The buckwheat seeds treated with different electric field intensity （0,3.0,4.0,5.0 and 6.0 kV/cm） for 15 rain were cultured in light incubator. The roots of seedlings with two leaves were treated using different concentrations of PEG for 48 h, and then the contents of proline, MDA and soluble sugar of the seedling leaves were determined. The result showed that the electric field strength of 4.0 kV/cm× 15 min and 5.0 kV/cm× 15 min could increase the contents of proline and soluble sugar, while decrease the content of MDA, so increase the drought resistance of buckwheat seedlings,indicating that the biological effect of electric field has a certain after-effect. The buck- wheat seedlings had different ability of drought resistance when treated with different electric field coditions,and the suitable electric fields were 4.0 kV/cm× 15 min and 5.0 kV/cm×15 min.