柞蚕雌蛾体外抗氧化活性研究

Antioxidant Activities of Antheraea pernyi Female Moth in Vitro

为探明柞蚕雌蛾的体外抗氧化活性,为其抗氧化药物的研制提供理论依据,将柞蚕雌蛾粉经80%乙醇超声提取后,测定柞蚕雌蛾提取液中总黄酮、总酚酸的含量,及其对超氧阴离子自由基、羟基自由基、1,1-二苯基-2-苦肼基(DPPH)自由基和2,2-连氮基-双-(3-乙基苯并二氢噻唑啉-6-磺酸)二铵盐(ABTS)自由基的清除作用。结果显示,柞蚕雌蛾中总黄酮含量为2.36mg/g,总酚酸含量为2.44mg/g;其具有较强的清除自由基能力,对超氧阴离子自由基、羟基自由基、DPPH自由基和ABTS自由基的半数抑制质量浓度(IC50)分别为23.525 8、27.998 4、0.648 9、6.755 7mg/mL。表明柞蚕雌蛾体外具有较强的抗氧化活性。

To investigate antioxidant activities of Antheraea pernyi female moth in vitro, and reveal the theoretic basis for exploitation of Antheraea pernyi female moth in antioxidant drugs, the contents of total flavonoids,total phenols and the removal effect on oxygen free radical, hydroxyl free radical, DPPH free radical and ABTS free radical of 80 % ethanol extraction from Antheraea pernyi female moth by ultrasonics were detected with UV-visible spectrophotometer. The results showed that the contents of flavonoid and total phenolic acid were 2. 36 and 2.44 mg/g%Antheraea pernyi female moth had certain ability of scavenging free radicals, the 50% elimination values to oxygen free radical, hydroxyl free radical,DPPH free radical and ABTS free radical were 23. 525 8,27. 998 4,0. 648 9,6. 755 7 mg/mL, respectively. Antheraea pernyi female moth has strong anti-oxidation activity.