金雀异黄素对多囊卵巢综合征大鼠卵巢组织中Bcl-2及Bax mRNA表达的影响

Effects of Genistein on Expressions of Bcl-2 and Bax mRNA in Ovarian Tissue of Polycystic Ovary Syndrome Rats

本试验旨在探讨金雀异黄素(genistein,GEN)对多囊卵巢综合征(polycystic ovary syndrome,PCOS)大鼠卵巢组织中抗凋亡基因Bcl-2及促凋亡基因Bax mRNA表达的影响。将90只雌性Wistar大鼠随机分为6组:对照组,模型组,GEN低、中、高剂量组及雌激素组。按照胰岛素联合人体绒毛膜促性腺激素造模法建立PCOS试验动物模型,造模成功后,每组选择10只大鼠进行试验。剂量组分别给予5、10、20 mg/kg GEN,雌激素组给予0.5 mg/kg己烯雌酚,受试物灌胃给予,持续15 d,观察动情周期10 d。采用原位杂交法检测各组大鼠卵巢组织中Bcl-2和Bax mRNA表达量。同时测定大鼠卵巢组织中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性及丙二醛含量。结果表明:与模型组相比,GEN能提高大鼠卵巢组织中Bcl-2 mRNA表达量,降低Bax mRNA表达量,并且可调节卵巢组织中抗氧化酶活性,其作用效果与雌激素相似。GEN可以增强大鼠卵巢组织中Bcl-2 mRNA表达量,拮抗Bax mRNA表达量,推测这是GEN改善PCOS卵巢功能的作用途径之一。

This experiment was conducted to study the effects of genistein （GEN） on the expressions of anti- apoptotic gene Bcl-2 mRNA and pro-apoptotic gene Bax mRNA in ovarian tissue of polycystic ovary syndrome （PCOS） rats. Ninety rats were divided into six groups： control group, model group, GEN low dose group, GEN middle dose group, GEN high dose group and estrogen group, respectively. The insulin combined with human chorionic gonadotropin molding method was used to establish PCOS animal models. After modeling, ten rats were selected from each group to carry out the test. The rats in dose groups were given 5, 10 and 20 mg/kg GEN, respectively, and the rats in estrogen group were given 0.5 mg/kg diethylstilbestrol. All compounds were administered intragastric administration on 15 consecutive days and observed a estrous cycle of 10 days. The expressions of Bcl-2 and Bax mRNA in rat ovarian tissue were examined by in situ hybridization. The activities of superoxide dismutase, catalase and glutathione peroxidase, and malondialdehyde content in rat ovarian tissue were determined. The results showed as follows： compared with PCOS model group, GEN could raise the expression of Bcl-2 mRNA and weaken the expression of Bax mRNA. And GEN could regulate the activities of antioxidant enzymes in rat ovarian tissue, which was as the same as the estrogen. In conclu- sion, GEN can enhance the expression of Bcl-2 mRNA and reduce the expression of Bax mRNA in rat ovarian tissue. It is presumed that it is one of the mechanisms of GEN improving ovarian function of PCOS.