比例法和绝对浓度法对结核分支杆菌药物敏感性测定的对比研究

A comparative study of proportion method and absolute concentration method on drug susceptibility testing of M.tuberculosis

目的 对比研究比例法和绝对浓度法测定结核分支杆菌的药物敏感性。方法 按照中国防痨协会《结核病诊断细菌学检验规程》 ,对 10 4株结核分支杆菌分别采用比例法和绝对浓度法检测药物敏感性。结果 比例法及绝对浓度法对耐药病例检出率及耐多药结核 (MDR -TB)检出率分别为 84.6 %、5 0 .0 %及 5 3.8%、40 .4% ,(P <0 .0 1)。比例法对链霉素 (SM)、异菸肼(INH)、利福平 (RFP)、乙胺丁醇 (EMB)、对氨基水杨酸钠 (PAS)等敏感与绝对浓度法符合率分别为 88.2 %、10 0 .0 %、10 0 .0 %、95 .0 %、96 .8%。两种检测方法对SM、INH、RFP、EMB、PAS各药的符合率分别为 88.5 %、84.6 %、80 .8%、6 3.5 %、73.1%。结论比例法结果精确 ,具有可比性。

Objective To study the susceptibility to drugs of proportion method and absolute concentration method of M.tuberculosis(MTB).Methods According to the Laboratory Science Procedure of Diagnostic Bacteriology in Tuberculosis Published by Chinese Antituberculosis Association,drug sensitivity test(DST)was performed to 104 strains of MTB using proportion method and absolute concentration method at the same time.Results By proportion method and absolute concentration method,the rates of drug resistance and multidrug resistance of tuberculosis were 84.6%、50.0% and 53.8%、40.4%,respectively.It was signficantly higher in proportion method than that of absolute concentration method(P<0.01).The sensitivity accordance rates to Streptomycin(SM)、Isoniazid(INH)、Rifampicin(RFP),Ethambutol(EMB)and Para-aminosalicylic acid(PAS)of proportion method compared to absolute comcentrtion method were 88.2%、100.0%、95.0% and 96.8%,respectively.The accordance rates to SM、INH、RFP、EMB and PAS between two methods were 88.5%、84.6%、80.8%、63.5% and 73.1%,respectively,Conclusions The results of proportion method were accurate and could be compared.