响应面法优化桑葚多糖的超声波辅助提取工艺条件

Optimization of Ultrasonic-assisted Extraction of Polysaccharides from Fructus Mori by Response Surface Methodology

本研究利用响应面法优化桑葚多糖的超声波辅助提取工艺条件;选定提取温度、时间及水料比作为影响因素,以桑葚多糖提取率为评价指标,在单因素实验的基础上,通过3因素3水平Box-Behnken中心组合试验建立多糖提取率的二次多项式回归方程,研究超声提取时间、温度、水料比对桑葚多糖提取率的影响;结果显示最佳提取工艺条件为提取温度72℃、超声时间23.5 min、水料比27∶1(v∶m,mL/g),在该条件下多糖提取率预测值为17.80%,验证值为17.78±0.85%(n=3);此方法与传统水提取法相比具有省时、高效的优点,为桑葚多糖的后续研发提供实验基础。

In the present study, the conditions for ultrasonic-assisted extraction of polysaccharides from Fructus Mori were optimized by response surface analysis. On the basis of single-factor experiments, the relationship between main extrac-tion conditions including extraction time, the ratio of water to raw material and extraction temperature were modeled using a 3-factor,3-1evel Box-Behnken experimental design. The established model was analyzed by response surface methodolo-gy to obtain the optimum extraction conditions. The results showed that the optimum extraction conditions were obtained as follows：extraction time was 23.5 rain, the ratio of water to raw material was 27：1 （v： m, mL/g） and extraction tem- perature was 72 ℃. Under these conditions, the estimated and observed values of maximal extraction yield of polysaccha-rides were 17. g0% and 17.78 ± 0.85% （n = 3） ,respectively. The method had the advantage of time-saving and effi-cient compared with the traditional water extraction. The results reported in this study provided the experimental basis for the follow-up research and development of Fructus Mori polysaccharides.