星形细胞瘤中P53、VEGF及PCNA表达的相关性及其与预后的关系

Expressions of P53, vascular endothelial growth factor and proliferating cell number antigen in astrocytomas and prognosis of these patients

目的研究星形细胞瘤中P53、血管内皮因子（vEGF）及增殖细胞核抗原（PCNA）表达的相关性及其与预后的关系。方法免疫组化染色sP法检测梅州市人民医院自2002年5月至2010年5月首次手术切除的126例不同级别原发性星形细胞瘤标本P53、VEGF及PCNA的表达，分析其相关性及其预后意义。结果P53、VEGF及PCNA在不同级别的星形细胞瘤中表达不同，随着级别的提高而表达强度增加，差异有统计学意义（P〈0．05）；P53与PCNA、P53与VEGF之间表达呈正相关关系（r=0．608，P=0．000；r=0．432，P=0．001）。相同级别星形细胞瘤中，P53阳性表达患者预后较差，生存率比较差异有统计学意义（P〈0．05），VEGF及PCNA与预后无关。结论星形细胞瘤级别提高，P53、VEGF及PCNA的表达强度增加：P53可作为判断肿瘤预后的指标之一。

Objective To study the expressions of P53, vascular endothelial growth factor （VEGF） and proliferating cell nuclear antigen （PCNA） in astrocytomas and their relationships with prognosis of these patients. Methods Immunohistochemical SP method was employed to detect the P53, VEGF and PCNA expression in 126 cases of human brain astrocytomas of different grades, collected in our hospital from May 2002 to May 2010; their correlations and their significance in the prognosis in these patients were analyzed. Results P53, VEGF, and PCNA expressed differently in astrocytomas of different grades, enjoying a positive relation with grades （P〈0.05）; positive correlations of between P53 expression and both VEGF and PCNA expressions （r=0.608, P=-0.000; r=0.432, P=0.001）. In patients with the same grade of astrocytoma, those having positive P53 expression enjoyed poorer prognosis and lower survival rate with significant difference （P〈0.05）; while VEGF and PCNA expressions had no correlation with the progonosis. Conclusion The expressions of PCNA, VEGF and P53 are closely associated with grade of the astrocytomas, and only P53 expression is an indicator of poor prognosis.