骨髓间充质干细胞抑制活化小胶质细胞的增殖、吞噬及炎性因子的分泌

Bone marrow-derived mesenchymal stem cells inhibit proliferation, phagocytosis and proinflammatory factors secretion of activated microgltas

目的观察骨髓间充质干细胞对脂多糖刺激后小胶质细胞活性的影响，探索骨髓间充质干细胞治疗中枢神经系统损伤的可能机制。方法实验分为空白对照组、脂多糖刺激组、条件培养基组、脂多糖＋条件培养基组，用脂多糖作为炎症刺激因素，诱导SD大鼠原代小胶质细胞达到活化状态，然后用骨髓间充质干细胞条件培养基孵育这些活化的小胶质细胞。用CCK．8法检测小胶质细胞的增殖能力，用吞噬荧光乳胶微球实验检测小胶质细胞的吞噬功能。用Bio-PlexProAssays和格里斯试剂检测小胶质细胞分泌炎性因子的变化。结果条件培养基组和对照组相比，小胶质细胞增殖能力和吞噬能力被显著抑制，差异均有统计学意3L（P=0．000，P=-0．033）；与对照组相比。条件培养基组小胶质细胞分泌的炎性因子也显著减少，差异有统计学意义（p〈0．05）。结论骨髓间充质干细胞具有抑制小胶质细胞激活的能力，可据此作为一种治疗中枢神经系统损伤的有效工具。

Objective To investigate whether bone marrow-derived mesenchymal stem cells （BMSCs） influence the activity of microglias after lipopolysaccharide （LPS） stimulation and explore the mechanism of BMSCs in treating central nervous system injury, Methods The best stimulation LPS concentration for microglias secretion was determined; four groups, as blank control group, LPS stimulation group, BMSCs stimulation group and LPS＋BMSCs stimulation group, were chosen in our study. The proliferation of activated microglias was detected by CCK-8, phagocytosis of activated microglias was detected by phagocytosis of fluorescent microspheres experiment and pro-inflammatory factors secretion changes of activated microglias were observed by Bio-Plex Pro assays and griess reagent. Results As compared with those in the blank control group, the proliferation and phagocytosis of activated microglias in the BMSCs stimulation group were significantly inhibited （P=0.000, P=-0.033）; so as to the proinflammatory factors secretion of activated microglias in the BMSCs stimulation group （P〈0.05）. Conclusion BMSCs have the ability to control microglial activation, indicating that BMSCs could be a promising therapeutic tool for treatment of diseases associated with microglial activation.