结直肠息肉、结直肠腺瘤和结直肠癌患者外周血中Th17和Tc17细胞的变化

The Changes of Circulating Th17 and Tc17 Cells in Patients with Colorectal Polyp, Adenoma and Cancer

目的检测结直肠息肉、结直肠腺瘤和结直肠癌患者外周血中辅助性T细胞(Th)17与杀伤性T细胞(Tc)17细胞比例的变化,并分析两者的相关性。方法收集结直肠息肉17例、结直肠腺瘤34例和结直肠癌42例患者资料,另取17名健康志愿者为对照。流式细胞术检测患者和健康对照外周血中Th17和Tc17细胞比例;ELISA法检测血清中白细胞介素(IL)-17A和IL-23水平。结果结直肠腺癌组患者外周血中Th17和Tc17细胞比例以及IL-17A和IL-23水平最高,结直肠腺瘤及息肉组较高,对照组两种细胞比例及两因子水平最低,结直肠腺瘤组Tc17细胞比例高于结直肠息肉组,但两组Th17细胞比例、IL-17A和IL-23水平差异无统计学意义。相关性分析表明Th17和Tc17细胞呈正相关(rs=0.594)。结论在结直肠息肉或结直肠腺瘤向结直肠癌发展中,外周血Th17和Tc17细胞比例均明显增多,且两者呈正相关。

Objective To compare ratio of circulating Th17 over Tc17 cells in patients with colorectal polyp （CRP）, colorectal adenoma （CRA） and colorectal cancer （CRC）,and analyse their relationship. Methods The ratio of circulating Th17 over Tc17 cells in 93 patients（17 CRP, 34 CRA and 42 CRC）and 17 healthy donors were examined by flow cytometry;Serum levels of IL-17A and IL-23 were tested by ELISA. Results The ratio of circulating Th17 over Tc17 cells and the se-rum levels of IL-17A and IL-23 were the lowest in healthy control groups, medium in CRP and CRA groups and the highest in CRC groups （P＆lt;0.05）. The proportions of Tc17 cells in CRA groups were significantly higher compared to those in CRP groups,but no statistical significance of proportion of circulating Th17 cells as well as serum levels of IL-17A and IL-23 be-tween CRA and CRP groups was observed （P＆gt;0.05）. Further analysis showed that there was a positive correlation between proportions of circulating Th17 and Tc17 cells （rs=0.594, P＆lt;0.001）. Conclusion The proportions of circulating Th17 and Tc17 cells were positively related and the ratio of Th17 over Tc17 was markedly increased from CRP group or CRA group to CRC group.