间充质干细胞分化为心肌细胞的诱导方法研究进展

Progress in Condition of Mesenchymal Stem Cells Differentiation into Cardiomyocytes

间充质干细胞作为一种取材方便、易于分离培养、体外扩增快、免疫原性低的成体干细胞,具有自我更新和多向分化潜能,可在体内外不同的诱导条件下分化为心肌细胞,是理想的心肌再生治疗的种子细胞。本文综述了间充质干细胞分化为心肌细胞的诱导方法,包括化学试剂、中药制剂、机械力和电磁刺激、心肌环境因子、损伤组织条件培养、组织工程方法等,为其在心肌损伤性疾病尤其是心肌梗死治疗中的应用提供基础。

Mesenchymal Stem Cells （MSCs） are regarded as typical adult stem cells which have the ability of self-renewal and multilineage differentiation potential. They can be easily isolated, cultured and expanded, and they have low immunogenicity. Particularly, MSCs can be induced to differentiate into cardiomyocytes （CMs） in different conditions in vivo or in vitro, and therefore they are ideal seed cells in cellular therapy for heart disease. In this article the methods of inducing differentiation of MSCs into cardiomyocytes are reviewed, including chemical reagents, traditional Chinese medicines, mechanical stress and electromagnetic stimulation, myocardial microenvironment, injured tissue conditioned foundation for applying MSCs to therapy of heart diseases infarction. media, tissue engineering and so on, to provide caused by myocardial damage, especially myocardial