宁夏无果枸杞芽提取物对慢性温和不可预见性应激小鼠学习、记忆的影响

The Effect of Ningxia Fruitless Chinese Wolfberry Bud Extracts on Learning and Memory in Chronic Mild Unpredictable Stress Mice

目的:探讨宁夏无果枸杞芽提取物(fruitless lycium-sprout extracts,FLE)对成年慢性应激小鼠学习、记忆功能,血清和脑组织中超氧化物歧化酶(SOD)的活性、丙二醛(MDA)含量及脑组织中神经细胞粘附分子(NCAM)表达的影响。方法:将40只成年昆明小鼠随机分为正常对照组、模型组、FLE低剂量组(FLE1组)和高剂量组(FLE2组),每组10只;采用慢性温和不可预见性应激(chronic mild unpredictable stress,CUMS)方法建立小鼠抑郁模型。在造模完成后灌胃给药,1次/d,连续灌胃6周,FLE1组、FLE2组分别用质量体积百分比为10%、20%的FLE每日0.2 ml/10 g灌胃,正常对照组及模型组灌胃等量生理盐水。用Morris水迷宫实验检测各组小鼠的学习记忆能力,黄嘌呤氧化酶法测定血清和脑组织SOD活性,硫代巴比妥酸法测定MDA水平,免疫组化染色法检测脑组织中N-CAM蛋白的表达。结果:与正常对照组比较,模型组小鼠的目标象限停留时间减少,隐藏平台象限获得时间延长,血清SOD活性降低,MDA水平提高,脑组织中N-CAM表达下调(P<0.01);与模型组比较,FLE2组的目标象限停留时间延长,隐藏平台象限获得时间缩短,血清和脑组织中SOD活性均增加,MDA含量均降低(P<0.05),脑组织中N-CAM表达均上调(P<0.001)。结论:FLE可以提高CUMS小鼠学习和记忆的能力,其机制可能与抗氧化作用有关。

Objective： To explore the effects of fruitless lycium-sprout extracts （FLE） of Ningxia Fruitless Chinese Wolfberry bud on learning and memory, SOD （superoxide dismutase） activity and MDA （methane dicarboxylic aldehyde） content in serum and brain tissue, as well as the expression of N-CAM in the cerebra cortex in C.UMS （chronic mild un- predictable stress） mice. Methods： 40 adult Kunming mice were randomly divided into model group, FLE in low dose group （group FLEI ） and high dose group （group FLE2）, 10 rats in each group, the other 10 KM mice as normal control group. In group FLE1 and group FLE2, the mice received intragastric administration with a concentration of 20% and 10% FLE respectively, 0.2 ml/10 g at day, continuous $ weeks. The normal control group and the model group were treated with intragastric saline. Using the Morris water maze experiment, the ability of learning and memory was exam- ined, SOD activity in serum and brain tissue was detected by xanthine oxidase assay, and MDA levels were evaluated by thiobarbituric acid method. Immunohistochemistry SP method was also used to detect the expression of N-CAM in brain tissue of mice. Results： Compared with the normal control group, the time in the target quadrant was reduced in model group mice, the time in hidden platform quadrant was extended, the activity of SOD in serum was decreased, the level of MDA was increased and the expression of N-CAM in brain was decreased （P 〈0.01 ）. Compared with the CUMS model group, the time in the target quadrant was extended, the time in hidden platform quadrant was reduced, the learning and memory abilities were improved in group FLE2, the activity of SOD in serum and brain tissue was increased, the content of MDA was decreased, and the expression of N-CAM in brain was increased （P 〈 0.05）. Conclusion： FLE could improve the ability of learning and memory in the CUMS mice, and its mechanism may be related to FLE antioxidant.