从溃疡性结肠炎大鼠肺组织炎性细胞因子及氧自由基表达的变化看肺与大肠相表里

Exploration of the Theory of "Fei and Dachang Being Interior-Exteriorly Related" from Observing Changes of Inflammatory Cytokines and Oxygen Free Radicals in the Lung Tissue of Ulcerative Colitis Rats

目的通过观察溃疡性结肠炎(ulcerative colitis,UC)大鼠肺组织中炎性细胞因子及氧自由基表达变化,探讨"肺与大肠相表里"理论及UC肺损伤的病理机制。方法健康雄性Wistar大鼠50只,随机选择其中25只采用结肠组织致敏与三硝基苯磺酸(TNBS)/乙醇相结合的免疫复合法复制大鼠UC模型(模型组),另25只设为正常对照组(正常组)。造模后第2、4周时观察大鼠肺组织病理形态学改变,ELISA法检测肺组织中肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)含量,比色法测定肺组织中超氧化物歧化酶(SOD)、丙二醛(MDA)活性。结果造模第2、4周时,模型组肺组织病理均表现异常,且与正常组比较,其肺组织中TNF-α、IL-1β、MDA含量均明显升高(P<0.01),SOD活性显著降低(P<0.05,P<0.01)。结论 TNF-α、IL-1β、SOD、MDA可能是UC肠病及肺的共同物质基础,为"肺与大肠相表里"理论提供了一定的现代医学实验依据。

Objective To explore the theory of ＂Fei and Dachang being interior-exteriorly related＂ and the pathogenesis of lung injury by observing changes of inflammatory cytokines and oxygen free radicals in ulcerative colitis （UC） rats. Methods Totally 50 healthy male Wistar rats were randomly divided into two groups, the normal control group and the model group, 25 rats in each group. The UC model was established by allergizing colon mucosa combined with TNBS-alcohol （50%） enema. Another 25 rats were recruited as the normal control group. At week 2 and 4 after modeling, the pathomorphological changes of the lung were ob- served. Furthermore, the contents of tumor necrosis factor oL （TNF-α） and IL-1β were determined by ELISA. The activities of superoxide dismutase （SOD） and malondialdehyde （MDA） were evaluated with colorimetry. Results Compared with the normal control group, the pathomorphology of the lung tissue in the model group appeared abnormal at week 2 and 4. Compared with the normal control group, levels of TNF-α, IL-1β, and MDA in the lung tissue significantly increased in the model group （P 〈 0. 01 ） and the activities of SOD significantly decreased （P 〈 0. 05, P 〈0. 01 ）. Conclusion TNF-α, IL-1β, SOD, and MDA might be common material bases for the large intestine involved in lung disease of UC patients, thus providing a modern scientific basis for the theory of Fei and Dachang being interior-exteriorly related.