大肠癌患者外周血CK19和MUC-1 mRNA水平及其临床意义

Expression of CK19 and MUC-1 mRNA in the peripheral blood of patients with colorectal cancer and their clinical significances

目的探讨大肠癌(CRC)患者外周血中角蛋白(CK)19、黏蛋白(MUC)-1 mRNA水平并分析两者与临床病理特征的关系。方法采用巢式逆转录聚合酶链式反应(RT-PCR)检测20例健康体检者(健康组)、20例大肠良性腺瘤患者(良性疾病组)及90例CRC患者(CRC组)的外周血CK19和MUC-1 mRNA水平,并分析两者与CRC临床病理参数(Dukes分期、分化程度、远处转移及组织学类型)的关系。结果 CRC组CK19 mRNA阳性率为58.9%(53/90),均高于健康组的0和良性疾病组的5.0%(1/20),差异有统计学意义(P<0.05);CRC组MUC-1 mRNA阳性率为52.2%(47/90),与健康组的60.0%(12/20)和良性疾病组的50.0%(10/20)比较,差异无统计学意义(P>0.05);健康组和良性疾病组CK19 mRNA、MUC-1mRNA阳性率的差异无统计学意义(P>0.05)。CRC中CK19 mRNA和MUC-1 mRNA阳性率均与Dukes分期、分化程度及远处转移有关(P<0.05),但与组织学类型无关(P>0.05)。结论 CRC患者外周血中CK19 mRNA阳性率明显升高,且外周血中CK19mRNA、MUC-1 mRNA与Dukes分期、分化程度及远处转移有关,提示两者可能在CRC发生发展中均有重要意义。

Objective To explore the expression of cytokeratin （ CK） 19 and mucin （ MUC）-1 mRNA in the peripheral blood of patients with colorectal cancer （ CRC） and their relationship with clinicopathological parameters. Methods The levels of CK19 and MUC-1 were measured by nested reverse transcription-polymerase chain reaction （ RT-PCR） in the peripheral blood of 20 healthy hu-man volunteers （ Healthy group ） , 20 patients with benign colorectal adenomas （ Benign disease group ） and 90 patients with CRC （ CRC group） . The correlations between levels of CK19 and MUC-1 mRNA and clinicopathological parameters of CRC （ Dukes stage, differentiation, distant metastasis and histological type） were analyzed. Results The positive rate of CK19 mRNA was 58？9% （ 53/90） of CRC group, higher than 0 of Healthy group and 5？0% （ 1/20） of Benign disease group with significant difference （ P〈0.05） . The positive rates of MUC-1 mRNA were 52？2% （ 47/90） , 60.0% （ 12/20） and 50？0% （ 10/20） for CRC group, Healthy group and Benign disease group with no significant difference （ P〉0,05） . No significant differences were observed between Healthy group and Be-nign disease group on positive rates of CK19 mRNA and MUC-1mRNA. The positive rates of CK19 mRNA and MUC-1 mRNA were re-lated with Dukes stage, differentiation and distant metastasis, and non-related with histological type （P〈0？05）. Conclusion The positive rate of CK19 mRNA in CRC increased, and CK19 and MUC-1 mRNA related with the Dukes stage, differentiation and distant metastasis, which may play an important role in the development of CRC.