FLNA蛋白在肾癌中的表达及其临床意义

Expression and clinical significance of FLNA in renal cell carcinoma

目的探讨细丝蛋白A(FLNA)在肾癌中的表达情况及其与临床病理特征的关系。方法分别采用免疫组化SP法、Western blotting检测70例肾癌组织及38例癌旁组织的FLNA蛋白阳性表达率和表达水平,分析肾癌组织中FLNA蛋白表达与临床病理参数(性别、年龄、肿瘤直径、病理类型、淋巴结转移、临床分期及病理分级)的关系。结果肾癌组织FLNA蛋白阳性表达率和表达水平分别为37.1%(26/70)和0.27±0.04,均低于癌旁组织的71.1%(27/38)和0.83±0.08,差异有统计学意义(P<0.05);FLNA蛋白表达与淋巴结转移、临床分期及病理分级有关(P<0.05);FLNA蛋白表达与年龄、性别、肿瘤直径及病理类型均无关(P>0.05)。结论肾癌组织中FLNA蛋白呈低表达,且与淋巴结转移、临床分期及病理分级有关,可能在肾癌的发生发展中发挥重要作用。

Objective To investigate the expression and clinical significance of filamin A（FLNA）in renal cell carcinoma. Methods The immunohistochemistry（ SP method） and Western blotting were used to analyze the positive expression rate and level of FLNA on tissue sections from 70 renal cell carcinoma tissues and 38 adjacent tissues. Then the relationships between FLNA expression and clinicopathological variables（ sex, age, tumor size, histological type, lymph node metastasis, clinical stage and histological grade） were analyzed. Results The positive expression rate and level of FLNA in renal cell carcinoma tissues were 37.1%（ 26/70） and 0.27 ＆#177;0.04, lower than 71.1%（27/38）and 0.83＆#177;0.08 in adjacent tissues with significant difference（P＆lt;0.05）. The FLNA expression is related with lymph node metastasis, clinical stage and histological grade（ P＆lt;0.05） , but not with sex, age, tumor size and histological type（P＆gt;0.05）. Conclusion There was a decreased expression rate and level of FLNA protein in renal cell carcinoma tissues, and its expression was related with lymph node metastasis, clinical stage and histological grade, indicating that FLNA may play an important role in the development of renal cell carcinoma.