多氯联苯对胎鼠神经干细胞Nnat基因表达的影响

Effect of Polychorinated Biphenyls on Expression of Neuronatin in Neural Stem Cells of Embryonic Mice

目的探讨环境污染物多氯联苯Aroclor 1254对神经发育损伤的作用机制.方法不同剂量的Aroclor 1254(A1254)作用于胎鼠的神经干细胞(NSC)72 h,通过实时定量RT-PCR检测Neuronatin(Nnat)mRNA的表达,Western blot检测Nnat蛋白质含量和流式细胞技术检测细胞凋亡情况.结果 (1)免疫荧光显示所培养细胞表达Nestin,且能进一步被诱导分化;(2)随着A1254剂量增加,Nnat mRNA和蛋白质表达水平下降,NSC细胞凋亡增加,差异有统计学意义(P<0.05).结论多氯联苯可能通过下调Nnat基因表达,神经细胞凋亡增加,导致胎儿的神经发育损伤.

Objective To study the mechanism of Polychorinated Biphenyls Aroclor 1254 in neural injury during nervous system development. Methods Neural stem ceils were treated for 72 h with different doses of Polychorinated Biphenyls Aroclor 1254. The mRNA expressions of Neuronatin were measured by real time RT-PCR. The protein expressions of Neuronatin were measured by the Western blot. Cell apoptosis were detected by flow cytometry. Results （1） The expression of Nestin was detected on the cells by immunocytofluorescence. （2） The mRNA and protein expression of Nnat in NSC were decreased, the NSC apoptosis was increased, and they displayed dose-depenence with A1254. Conclusion Polychorinated Biphenyls can cause nervous injury, its mechanism maybe related to the down-regulation of the expression of Nnat and the promotion of the apoptosis.