单核细胞趋化蛋白-1趋化巨噬细胞迁移与侵袭的体外实验研究

Experimental Study on Monocyte Chemoattractant Protein 1 for the Migration and Invasion of Macrophage in Vitro

目的探讨单核细胞趋化蛋白-1（MCP-1）对巨噬细胞（NR8383）体外迁移和侵袭作用的影响．方法体外传代培养NR8383细胞，免疫组化检测NR8383细胞上MCP一1特异性受体（CCR2）的表达，细胞迁移和Transwell小室侵袭实验分别观察MCP-1及CCR2拮抗剂（RS504393）对NR8383细胞迁移和侵袭作用的影响．结果免疫组化检测显示NR8383细胞膜上存在有CCR2表达；细胞迁移和侵袭实验证明MCP-1促进NR8383细胞迁移和侵袭，而RS504393抑制NR8383细胞迁移和侵袭；不同浓度MCP-1（200～800μg/L）诱导下，NR8383细胞迁移能力随MCP-1浓度增高而增强（P〈0．05）．结论MCP-1对巨噬细胞迁移和侵袭具有促进作用，其作用与MCP-1受体CCR2结合有关．

Objective To explore the effect of monocyte chemoattractant protein -1 （MCP-1） on the migration and invasion of macrophage in vitro. Methods NR8383 macrophages were subcuhured in vitro.The expression of CCR2 （the receptor of MCP-1） in macrophage was detected by immunohistochemistry, The effect of MCP-1 and anti-CCR2 polyclonal antibodies （RS504393） on the migration and invasion of NR8383 macrophage were observed by agarose and transwell chamber assay. Results CCR2 was expressed in membrane of NR8383 macrophage by immunohischemical assessment. The cell migration and invasion experiments confirmed that MCP-1 induced the migration and invasion of NR8383 macrophage, but RS504393 inhibited the migration and invasion of NR8383 macrophage. The migration of macrophage was enhanced with the MCP-1 concentration. Conclusion MCP-1 can accelerate the migration and invasion of macrophage, which may be associated with MCP-1 receptor CCR2 binding.